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线性分子式:
O2NC6H4OP(O)(ONa)2 · 6H2O
化学文摘社编号:
分子量:
371.14
NACRES:
NA.83
PubChem Substance ID:
UNSPSC Code:
12352204
EC Number:
224-246-5
MDL number:
Beilstein/REAXYS Number:
5324661
eCl@ss:
32160703
产品名称
4-硝基苯基磷酸盐 二钠盐 六水合物, tablet
form
tablet
Quality Segment
technique(s)
protein detection: suitable
solubility
water: 1 tablet/3 mL, clear, colorless
storage temp.
−20°C
SMILES string
O.O.O.O.O.O.[Na+].[Na+].[O-][N+](=O)c1ccc(OP([O-])([O-])=O)cc1
InChI
1S/C6H6NO6P.2Na.6H2O/c8-7(9)5-1-3-6(4-2-5)13-14(10,11)12;;;;;;;;/h1-4H,(H2,10,11,12);;;6*1H2/q;2*+1;;;;;;/p-2
InChI key
KAKKHKRHCKCAGH-UHFFFAOYSA-L
General description
用于测定酸性和碱性磷酸酶的显色底物。该反应产生黄色的可溶性最终产物。可以用 3N NaOH 终止反应,并在 405nm 下读数。
Application
4-Nitrophenyl phosphate disodium salt hexahydrate has been used in enzyme-linked immunosorbent assay (ELISA) as a substrate for streptavidin-Alkaline Phosphatase. It has also been used as a substrate in control assay for comparing 4-aminophenyl phosphate/diethanolamine (APP/DEA) assay for alkaline phosphatase.
Biochem/physiol Actions
4-Nitrophenyl phosphate disodium salt hexahydrate (pNPP) is a non-proteinaceous and non-specific substrate for determining protein tyrosine phosphatase activity. Hydrolysis of pNPP yields p-nitrophenol. pNPP is a common chromogenic substrate for alkaline phosphatase.
Preparation Note
Contains 15 mg substrate per tablet
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存储类别
11 - Combustible Solids
WGK
WGK 3
闪点 (F)
Not applicable
闪点 (°C)
Not applicable
PPE(个人防护设备)
Eyeshields, Gloves, type N95 (US)