产品名称
SIGMAFAST™ 对硝基苯基磷酸酯片, tablet, To prepare 20 mL
质量水平
表单
tablet
溶解性
water: 0.25 tablet/mL, clear, colorless
储存温度
−20°C
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应用
开发用作可溶性底物，用于检测酶免疫分析法（EIA）中碱性磷酸酶的活性。 pNPP是高灵敏度碱性磷酸酶EIA检测的首选底物。将一片pNPP和一片Tris缓冲片溶解在去离子水中即得即用型底物。 SIGMAFAST pNPP每片可产生含有1.0 mg/ml pNPP、0.2 M Tris缓冲液的溶液。使用pNPP的EIA应用可以在定时实验中读数，或用3 N NaOH终止反应后延迟读数。SIGMAFAST™ 仅需要添加蒸馏水或去离子水即可制备活性底物溶液。片剂用箔包单个包装。
制备说明
将每片片剂溶于 20 mL 去离子水中，得到含 pNPP 的即用型缓冲液。
法律信息
SIGMAFAST is a trademark of Sigma-Aldrich Co. LLC
储存分类代码
11 - Combustible Solids
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
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商品
NBT-BCIP substrate system aids in western blotting and immunohistological staining, producing a blue-purple insoluble end product.
硝基蓝四唑（NBT）可与碱性磷酸酶底物5-溴-4-氯-3-吲哚基磷酸盐（BCIP）一起用于蛋白印迹和免疫组织染色程序。这些底物系统产生不溶的NBT二甲终产物，其颜色为蓝至紫色，可以通过视觉观察到。
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