Monoclonal Anti-Nedd8 antibody produced in mouse

Monoclonal Anti-Nedd8 (mouse IgG2b isotype) is derived from the ND23 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic peptide corresponding to amino acids of human Nedd8 conjugated keyhole limpet hemocyanin (KLH). Nedd8 is a small protein (9 kDa) that shows the highest similarity to the ubiquitin protein (60% amino acid sequence identity).

Neural precursor cell expressed, developmentally down-regulated 8 (NEDD8) is an ubiquitin-like, 76 amino acid protein. It is expressed in endocrine and epithelial cells. NEDD8 belongs to the ubiquitin-like protein superfamily.

synthetic peptide corresponding to amino acids 20-32 of human Nedd8, conjugated to KLH.

Monoclonal Anti-Nedd8 antibody produced in mouse has been used in:

• enzyme-linked immunosorbent assay (ELISA)
• immunoblotting
• immunocytochemistry

Nedd8 conjugation has a physiological role in processes where the stability of nuclear factor kappa light chain enhancer of activated B cells (NF-κB) and p27 is important.

Neural precursor cell expressed, developmentally down-regulated 8 (NEDD8) is involved in pathways concerned with protein degradation. It targets substrates for neddylation, which is a post-translational modification. NEDD8 also regulates the signaling of proteins like cullins.

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

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