Monoclonal Anti-Neurofilament 160 antibody produced in mouse

Monoclonal Anti- Neurofilament 160 (mouse IgG1 isotype) is derived from the hybridoma RMO44 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with purified mid-sized rat neurofilament (NF-M) subunit. Neurofilaments are one of the five major groups of intermediate filaments (Ifs) and are found predominantly in cells or tissues of neuronal origin. They are composed of three major proteins of apparent molecular weights 68 kDa, 160 kDa and 200 kDa.

Neurofilament 160 (NEFM) is one among the most abundant filaments in post-mitotic neurons.

purified mid-sized rat neurofilament (NF-M) subunit.

Monoclonal Anti-Neurofilament 160 antibody produced in mouse has been used in:

• enzyme-linked immunosorbent assay (ELISA)
• western blot analysis
• immunohistochemistry
• antibody labelling

Neurofilament proteins are synthesized in the neuronal perikarya, assembled to form filaments and then slowly transported within the axons towards the synaptic terminals. These molecules undergo post-translational modifications, which results in their heterogeneity including different levels of phosphorylation. The phosphorylation of neurofilament polypeptides has been suggested to modulate their function by influencing their interaction with cytoplasmic organelles.

Neurofilament 160 (NEFM) associates with the dopamine receptor.

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

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