N3129
4-硝基苯磷酸盐 双环己铵盐
phosphatase substrate, chromogenic, ≥97% anhydrous basis, powder
别名:
p-Nitrophenyl phosphate bis(cyclohexylammonium) salt, PNPP
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关于此项目
线性分子式:
O2NC6H4OP(O)(O-)2 · 2C6H11NH3+
化学文摘社编号:
分子量:
417.44
NACRES:
NA.83
PubChem Substance ID:
UNSPSC Code:
12352204
EC Number:
257-949-0
MDL number:
Beilstein/REAXYS Number:
6553276
产品名称
4-硝基苯磷酸盐 双环己铵盐, phosphatase substrate
Quality Segment
assay
≥97% anhydrous basis
form
powder
solubility
water: 10 mg/mL, clear, colorless to faintly yellow
storage temp.
−20°C
SMILES string
NC1CCCCC1.NC2CCCCC2.OP(O)(=O)Oc3ccc(cc3)[N+]([O-])=O
InChI
1S/C6H6NO6P.2C6H13N/c8-7(9)5-1-3-6(4-2-5)13-14(10,11)12;2*7-6-4-2-1-3-5-6/h1-4H,(H2,10,11,12);2*6H,1-5,7H2
InChI key
KXNNUYCXODNFBD-UHFFFAOYSA-N
Application
4-Nitrophenyl phosphate bis(cyclohexylammonium) salt has been used as a component in the colorimetric reagent for abscisic acid (ABA) assay.
Biochem/physiol Actions
p-Nitrophenyl phosphate (pNPP) is the preferred substrate for use with alkaline phosphatase in enzyme-linked immunosorbent assay (ELISA) procedures. Due to its high sensitivity, pNPP is the considered substrate in alkaline phosphatase systems. ELISA applications based on pNPP can be read in timed assays or delayed readings by stopping with alkaline solutions. This substrate yields a yellow-colored soluble end product that can be measured spectrophotometrically at 405 nm.
存储类别
11 - Combustible Solids
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)