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Merck
CN

N3129

Sigma-Aldrich

4-硝基苯磷酸盐 双环己铵盐

phosphatase substrate, chromogenic, ≥97% anhydrous basis, powder

别名:

p-Nitrophenyl phosphate bis(cyclohexylammonium) salt, PNPP

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关于此项目

线性分子式:
O2NC6H4OP(O)(O-)2 · 2C6H11NH3+
化学文摘社编号:
52483-84-8
分子量:
417.44
Beilstein:
6553276
EC 号:
257-949-0
MDL编号:
MFCD00013173
UNSPSC代码:
12352204
PubChem化学物质编号:
24897623
NACRES:
NA.83

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产品名称

4-硝基苯磷酸盐 双环己铵盐, phosphatase substrate

方案

≥97% anhydrous basis

表单

powder

溶解性

water: 10 mg/mL, clear, colorless to faintly yellow

储存温度

−20°C

SMILES字符串

NC1CCCCC1.NC2CCCCC2.OP(O)(=O)Oc3ccc(cc3)[N+]([O-])=O

InChI

1S/C6H6NO6P.2C6H13N/c8-7(9)5-1-3-6(4-2-5)13-14(10,11)12;2*7-6-4-2-1-3-5-6/h1-4H,(H2,10,11,12);2*6H,1-5,7H2

InChI key

KXNNUYCXODNFBD-UHFFFAOYSA-N

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相关类别

应用

4-Nitrophenyl phosphate bis(cyclohexylammonium) salt has been used as a component in the colorimetric reagent for abscisic acid (ABA) assay.

生化/生理作用

p-Nitrophenyl phosphate (pNPP) is the preferred substrate for use with alkaline phosphatase in enzyme-linked immunosorbent assay (ELISA) procedures. Due to its high sensitivity, pNPP is the considered substrate in alkaline phosphatase systems. ELISA applications based on pNPP can be read in timed assays or delayed readings by stopping with alkaline solutions. This substrate yields a yellow-colored soluble end product that can be measured spectrophotometrically at 405 nm.

储存分类代码

11 - Combustible Solids

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves, type N95 (US)

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
0000504896
0000504896
0000501319
0000501321
0000501321

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Brittany R Jenkins et al.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 35(6), e21551-e21551 (2021-05-28)
Intestinal epithelial cells (IEC) are crucial for maintaining proper digestion and overall homeostasis of the gut mucosa. IEC proliferation and differentiation are tightly regulated by well described pathways, however, relatively little is known about how cytokines shape these processes. Given
Abscisic acid synthesis in Acer rubrum L. leaves?a vapor-pressure-deficit-mediated response
Bauerle WL, et al.
J. Am. Soc. Hort. Sci., 129(2), 182-187 (2004)
Leaf abscisic acid accumulation in response to substrate water content: Linking leaf gas exchange regulation with leaf abscisic acid concentration
Bauerle WL, et al.
J. Am. Soc. Hort. Sci., 131(2), 295-301 (2006)
Ziad Dahabreh et al.
World journal of stem cells, 6(4), 497-504 (2014-09-27)
To compare seven commercially available bone graft substitutes (BGS) in terms of these properties and without using any additional biological growth factors. Porcine osteoprogenitor cells were loaded on seven commercially available BGS and allowed to proliferate for one week followed
Bjarni Ásgeirsson et al.
Biochemistry and biophysics reports, 24, 100830-100830 (2020-10-27)
Para-nitrophenyl phosphate, the common substrate for alkaline phosphatase (AP), is available as a cyclohexylamine salt. Here, we report that cyclohexylamine is a non-competitive inhibitor of APs. Cyclohexylamine inhibited four different APs. Co-crystallization with the cold-active Vibrio AP (VAP) was performed

商品

Colorimetric Phosphatase/Peroxidase Substrate Systems

NBT-BCIP substrate system aids in western blotting and immunohistological staining, producing a blue-purple insoluble end product.

比色碱性磷酸酶和过氧化物酶底物检测系统

硝基蓝四唑（NBT）可与碱性磷酸酶底物5-溴-4-氯-3-吲哚基磷酸盐（BCIP）一起用于蛋白印迹和免疫组织染色程序。这些底物系统产生不溶的NBT二甲终产物，其颜色为蓝至紫色，可以通过视觉观察到。

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