一般描述
这种中和缓冲液B要与 Sigma′REDExtract-N-Amp™ 组织 PCR.试剂盒一起使用。该缓冲液用于中和DNA提取液，并与REDExtract-N-Amp PCR 反应混合液及PCR引物一起用于扩增靶标DNA。 REDExtract-N-Amp试剂盒包括足够体积的中和缓冲液B，每次提取至少可中和一次。 不过，如果需要更多的扩增反应，则可能需要补充额外的中和缓冲液。
应用
中和缓冲液B 已用于改良 Sigma Extract-N-Amp™提取程序。它还用于小鼠的基因分型，在聚合酶链反应 (PCR) 之前可使用Sigma的REDExtract-N-Amp™ PCR 反应混合物来中和DNA提取液。
法律信息
Extract-N-Amp is a trademark of Sigma-Aldrich Co. LLC
REDExtract-N-Amp is a trademark of Sigma-Aldrich Co. LLC
警示用语：
Warning
危险分类
Aquatic Chronic 2 - Eye Irrit. 2 - Skin Irrit. 2 - Skin Sens. 1
储存分类代码
12 - Non Combustible Liquids
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
含少量动物源组分生物产品
历史批次信息供参考:
分析证书(COA)
Lot/Batch Number
Işın Çakır et al.
Nature metabolism, 4(1), 44-59 (2022-01-19)
The adipose tissue-derived hormone leptin can drive decreases in food intake while increasing energy expenditure. In diet-induced obesity, circulating leptin levels rise proportionally to adiposity. Despite this hyperleptinemia, rodents and humans with obesity maintain increased adiposity and are resistant to
Yan Zhang et al.
American journal of physiology. Renal physiology, 319(6), F1135-F1148 (2020-11-10)
Autosomal dominant polycystic kidney disease (ADPKD) is characterized by the presence of numerous fluid-filled cysts, extensive fibrosis, and the progressive decline in kidney function. Transforming growth factor-β1 (TGF-β1), an important mediator for renal fibrosis and chronic kidney disease, is overexpressed
A high-throughput functional genomics workflow based on CRISPR/Cas9-mediated targeted mutagenesis in zebrafish
Varshney GK, et al.
Nature Protocols, 11(12), 2357-2357 (2016)
Jun He et al.
Pharmacology, biochemistry, and behavior, 86(2), 327-333 (2006-12-16)
Adolescence is an important stage of brain development. Recent studies have indicated that neurogenesis in the brain occurs throughout life prompting comparisons of adolescent and adult neurogenesis. Since insulin-like growth factor 1 (IGF-1) has been implicated in promoting neurogenesis we
Yan Zhang et al.
American journal of physiology. Renal physiology, 319(6), F1135-F1148 (2020-11-10)
Autosomal dominant polycystic kidney disease (ADPKD) is characterized by the presence of numerous fluid-filled cysts, extensive fibrosis, and the progressive decline in kidney function. Transforming growth factor-β1 (TGF-β1), an important mediator for renal fibrosis and chronic kidney disease, is overexpressed
实验方案
SYBR® Green Extract-N-Amp™ Tissue PCR Kit enables rapid genomic DNA extraction and amplification from animal tissues.
我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
联系客户支持