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Key Documents

安全信息

N3910

Sigma-Aldrich

中和液 B

别名:

DNA neutralization solution, neutralization buffer DNA extraction

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About This Item

UNSPSC代码:
12161703
NACRES:
NA.25

等级

for molecular biology

形式

liquid

技术

DNA amplification: suitable
DNA extraction: suitable

储存温度

−20°C

一般描述

这种中和缓冲液B要与 Sigma′REDExtract-N-Amp 组织 PCR.试剂盒一起使用。该缓冲液用于中和DNA提取液,并与REDExtract-N-Amp PCR 反应混合液及PCR引物一起用于扩增靶标DNA。 REDExtract-N-Amp试剂盒包括足够体积的中和缓冲液B,每次提取至少可中和一次。 不过,如果需要更多的扩增反应,则可能需要补充额外的中和缓冲液。

应用

中和缓冲液B 已被用于改良的 Sigma Extract-N-Amp 提取程序。它也用于小鼠的基因分型,用于聚合酶链反应 (PCR) 之前使用Sigma′的REDExtract-N-Amp PCR 反应混合物来中和DNA提取液。

法律信息

Extract-N-Amp is a trademark of Sigma-Aldrich Co. LLC
REDExtract-N-Amp is a trademark of Sigma-Aldrich Co. LLC

象形图

Exclamation markEnvironment

警示用语:

Warning

危险分类

Aquatic Chronic 2 - Eye Irrit. 2 - Skin Irrit. 2 - Skin Sens. 1

储存分类代码

12 - Non Combustible Liquids

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

法规信息

含少量动物源组分生物产品

分析证书(COA)

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访问文档库

Yan Zhang et al.
American journal of physiology. Renal physiology, 319(6), F1135-F1148 (2020-11-10)
Autosomal dominant polycystic kidney disease (ADPKD) is characterized by the presence of numerous fluid-filled cysts, extensive fibrosis, and the progressive decline in kidney function. Transforming growth factor-β1 (TGF-β1), an important mediator for renal fibrosis and chronic kidney disease, is overexpressed
Işın Çakır et al.
Nature metabolism, 4(1), 44-59 (2022-01-19)
The adipose tissue-derived hormone leptin can drive decreases in food intake while increasing energy expenditure. In diet-induced obesity, circulating leptin levels rise proportionally to adiposity. Despite this hyperleptinemia, rodents and humans with obesity maintain increased adiposity and are resistant to
Yan Zhang et al.
American journal of physiology. Renal physiology, 319(6), F1135-F1148 (2020-11-10)
Autosomal dominant polycystic kidney disease (ADPKD) is characterized by the presence of numerous fluid-filled cysts, extensive fibrosis, and the progressive decline in kidney function. Transforming growth factor-β1 (TGF-β1), an important mediator for renal fibrosis and chronic kidney disease, is overexpressed
A high-throughput functional genomics workflow based on CRISPR/Cas9-mediated targeted mutagenesis in zebrafish
Varshney GK, et al.
Nature Protocols, 11(12), 2357-2357 (2016)
Jun He et al.
Pharmacology, biochemistry, and behavior, 86(2), 327-333 (2006-12-16)
Adolescence is an important stage of brain development. Recent studies have indicated that neurogenesis in the brain occurs throughout life prompting comparisons of adolescent and adult neurogenesis. Since insulin-like growth factor 1 (IGF-1) has been implicated in promoting neurogenesis we

实验方案

SYBR® Green Extract-N-Amp™ Tissue PCR Kit enables rapid genomic DNA extraction and amplification from animal tissues.

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