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Merck
CN

N4787

Nek2, Active human

recombinant, expressed in E. coli, ≥85% (SDS-PAGE), buffered aqueous glycerol solution

别名:

Never In Mitosis Gene A-Related Kinase 2, Nima-Related Kinase 2

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关于此项目

UNSPSC Code:
51111800
NACRES:
NA.32
MDL number:
Assay:
≥85% (SDS-PAGE)
Recombinant:
expressed in E. coli
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recombinant

expressed in E. coli

Quality Segment

assay

≥85% (SDS-PAGE)

form

buffered aqueous glycerol solution

enzyme activity

≥10 units/mg protein

mol wt

protein apparent mol wt ~80 kDa

UniProt accession no.

shipped in

dry ice

storage temp.

−70°C

Gene Information

human ... NEK2(4751)

Application

Kinase activity is measured as the molar amount of phosphate incorporated into myelin basic protein, per minute per mg protein at 30 °C using a final concentration of 50 μM [32P] ATP.

Biochem/physiol Actions

Nek2 (nimA-related kinase 2) is a cell cycle-regulated protein kinase with maximal activity at the onset of mitosis that localizes to the centrosome.

Physical form

Solution in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM DTT, 0.1 mM EGTA, and 30% glycerol.

Other Notes

One unit will incorporate 1 nanomole of phosphate into myelin basic protein (MBP) per minute at pH 7.2 at 30 deg C using a final concentration of 50 μm (32p) ATP


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存储类别

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

法规信息

常规特殊物品

此项目有



历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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全球贸易项目编号

货号GTIN
N4787-5UG04061837906626