N5386
微球菌核酸酶 来源于金黄色葡萄球菌
100-300 units/mg protein
别名:
MNase, 微球菌核酸内切酶
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关于此项目
化学文摘社编号:
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-748-0
MDL number:
Specific activity:
100-300 units/mg protein
Biological source:
Staphylococcus aureus
产品名称
微球菌核酸酶 来源于金黄色葡萄球菌, 100-300 units/mg protein
biological source
Staphylococcus aureus
form
powder
specific activity
100-300 units/mg protein
composition
Protein, ≥40% E1%/280 (, Balance primarily sodium acetate)
technique(s)
DNA extraction: suitable
DNA purification: suitable
suitability
suitable for molecular biology
application(s)
cell analysis
storage temp.
−20°C
Quality Level
Gene Information
Staphylococcus aureus subsp. aureus Mu50 ... nuc(1120790)
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Application
微球菌核酸酶可用于消化染色质以鉴定核小体的位置。
来源于金黄色葡萄球菌的微球菌核酸酶已被用于研究中监测与 DNA 的杂交反应。它也被用于研究葡萄球菌核酸酶测定的最佳条件。
Biochem/physiol Actions
可水解DNA的5′-磷酸二酯键。
Other Notes
注意：1μmole单位 = 约85 A260 单位，其中A260单位相当于在pH 8.8，37℃条件下，在3.55 mL反应体积中，30分钟内ΔA260为1.0。光路= 1cm。
存储类别
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
法规信息
常规特殊物品
此项目有
Use of micrococcal nuclease to monitor hybridization reactions with DNA.
D L Kacian et al.
Analytical biochemistry, 58(2), 534-540 (1974-04-01)
Optimal conditions for assay of staphylococcal nuclease
KAMMAN, J. and S. TATINI
Journal of Food Science, 42, 421-424 (1977)
Julien Roche et al.
Journal of the American Chemical Society, 135(39), 14610-14618 (2013-08-31)
The time required to fold proteins usually increases significantly under conditions of high pressure. Taking advantage of this general property of proteins, we combined P-jump experiments with NMR spectroscopy to examine in detail the folding reaction of staphylococcal nuclease (SNase)
Gang Wei et al.
Methods in enzymology, 513, 297-313 (2012-08-30)
Gene transcription can be regulated through alteration of chromatin structure, such as changes in nucleosome positioning and histone-modification patterns. Recent development of techniques based on the next-generation sequencing technology has allowed high-resolution analysis of genome-wide distribution of these chromatin features.
Julien Roche et al.
Biochemistry, 51(47), 9535-9546 (2012-11-03)
The folding of staphylococcal nuclease (SNase) is known to proceed via a major intermediate in which the central OB subdomain is folded and the C-terminal helical subdomain is disordered. To identify the structural and energetic determinants of this folding free
相关内容
QC Methods
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