生物来源
Staphylococcus aureus
质量水平
表单
powder
比活
100-300 units/mg protein
组成
Protein, ≥40% E1%/280 (, Balance primarily sodium acetate)
技术
DNA extraction: suitable
DNA purification: suitable
适用性
suitable for molecular biology
应用
cell analysis
储存温度
−20°C
基因信息
Staphylococcus aureus subsp. aureus Mu50 ... nuc(1120790)
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应用
微球菌核酸酶可用于消化染色质以鉴定核小体的位置。
来源于金黄色葡萄球菌的微球菌核酸酶已被用于研究中监测与 DNA 的杂交反应。它也被用于研究葡萄球菌核酸酶测定的最佳条件。
生化/生理作用
可水解DNA的5′-磷酸二酯键。
其他说明
注意：1μmole单位 = 约85 A260 单位，其中A260单位相当于在pH 8.8，37℃条件下，在3.55 mL反应体积中，30分钟内ΔA260为1.0。光路= 1cm。
储存分类代码
11 - Combustible Solids
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, type N95 (US)
法规信息
常规特殊物品
此项目有
Use of micrococcal nuclease to monitor hybridization reactions with DNA.
D L Kacian et al.
Analytical biochemistry, 58(2), 534-540 (1974-04-01)
Ho-Ryun Chung et al.
PloS one, 5(12), e15754-e15754 (2011-01-06)
Eukaryotic genomes are packed into chromatin, whose basic repeating unit is the nucleosome. Nucleosome positioning is a widely researched area. A common experimental procedure to determine nucleosome positions involves the use of micrococcal nuclease (MNase). Here, we show that the
Optimal conditions for assay of staphylococcal nuclease
KAMMAN, J. and S. TATINI
Journal of Food Science, 42, 421-424 (1977)
Julien Roche et al.
Biochemistry, 51(47), 9535-9546 (2012-11-03)
The folding of staphylococcal nuclease (SNase) is known to proceed via a major intermediate in which the central OB subdomain is folded and the C-terminal helical subdomain is disordered. To identify the structural and energetic determinants of this folding free
Julien Roche et al.
Journal of the American Chemical Society, 135(39), 14610-14618 (2013-08-31)
The time required to fold proteins usually increases significantly under conditions of high pressure. Taking advantage of this general property of proteins, we combined P-jump experiments with NMR spectroscopy to examine in detail the folding reaction of staphylococcal nuclease (SNase)
相关内容
QC Methods
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