N6009
神经生长因子-2.5S 来源于鼠颔下腺
lyophilized powder, suitable for cell culture
别名:
NGF-2.5S
产品名称
神经生长因子-2.5S 来源于鼠颔下腺, NGF-2.5S, lyophilized powder, suitable for cell culture
biological source
mouse
form
lyophilized powder
potency
0.1-30 ng/mL EC50 (using PC-12 cells)
mol wt
26.5 kDa
packaging
pkg of 4X25 μg
storage condition
avoid repeated freeze/thaw cycles
technique(s)
cell culture | mammalian: suitable
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
Quality Level
Gene Information
mouse ... Ngf(18049)
Analysis Note
采用 3 天 MTT 试验测定生物活性。
Application
来自小鼠颌下腺的神经生长因子-2.5已用于：
- Ham′ F-12培养基中，以使用背根神经节（DRG）进行生长锥塌陷测试
- “工艺室”中进行微流控室研究的培养基补充剂
- 处理中期黄体细胞，以研究其对胆碱乙酰基转移酶（CHAT）表达的影响
Biochem/physiol Actions
神经生长因子（NGF）具有神经营养作用，可维持周围神经系统的交感神经元和感觉神经元以及前脑胆碱能神经元。NGF可促进来自鸡胚背根神经节和大鼠PC12嗜铬细胞瘤细胞的神经突样细丝的生长。NGF可能会在体内胎儿发育和神经再生中发挥作用，尤其在缺血、手术或化学损伤后。NGF也可能与中枢神经系统有关。它对溃疡（人体皮肤、角膜溃疡和压疮）、青光眼、黄斑病变和视网膜色素变性等具有改善作用。NGF-2.5S是一种最初在解离条件下产生的NGF-b，通常因其在纯化过程中会发生蛋白水解而与NGF-b有所不同。
General description
神经生长因子（NGF）由不同类型的细胞生成，例如结构细胞、辅佐细胞和免疫细胞等。它属于神经营养因子家族。
Other Notes
神经生长因子-7S（NGF-7S）的2.5S亚基在最初分离的条件下通过Bocchini和Angeletti方法的改进从雄性小鼠颌下腺中分离时基本上是β-亚基。
Physical form
从乙酸钠缓冲液中的0.2 μm滤过溶液冻干。
存储类别
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
法规信息
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