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N7653

对硝基苯磷酸盐液体底物系统

Name: <I>对</I>硝基苯磷酸盐液体底物系统
Brand: SIGMA

alkaline phosphatase substrate, liquid

别名:

4-硝基苯磷酸二钠溶液, pNPP

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关于此项目

线性分子式:

O₂NC₆H₄OP(O)(ONa)₂

化学文摘社编号:

4264-83-9

分子量:

263.05

UNSPSC Code:

12352204

PubChem Substance ID:

24897835

NACRES:

NA.83

MDL number:

MFCD00007319

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产品名称

对硝基苯磷酸盐液体底物系统, liquid

form

liquid

Quality Level

200

shipped in

wet ice

storage temp.

−20°C

SMILES string

[Na+].[Na+].[O-][N+](=O)c1ccc(OP([O-])([O-])=O)cc1

InChI

1S/C6H6NO6P.2Na/c8-7(9)5-1-3-6(4-2-5)13-14(10,11)12;;/h1-4H,(H2,10,11,12);;/q;2*+1/p-2

InChI key

VIYFPAMJCJLZKD-UHFFFAOYSA-L

General description

对硝基苯磷酸盐（pNPP）液体底物系统将对硝基苯磷酸盐、缓冲液和所需的镁阳离子结合在一起，成为一种便捷的即用型单一溶液试剂。pNPP能够产生可溶性终产物，是碱性磷酸酶免疫测定的理想底物。

Application

在碱性磷酸酶测定中，采用p-对硝基苯基磷酸盐液体底物体系作为测定碱性磷酸酶活性的底物。

碱性磷酸酶的底物，能够产生可在405 nm处读取的可溶性黄色终产物。使用3N NaOH可终止反应并在405 nm处读取结果。推荐用于ELISA（微孔）分析，不建议用于膜应用。

Biochem/physiol Actions

4-硝基苯基磷酸二钠盐六水(pNPP)是测定蛋白质酪氨酸磷酸酶活性的非蛋白质和非特异性底物。pNPP水解后会生成p-硝基酚。它是碱性磷酸酶常见的显色底物。

Physical form

即用型。

Disclaimer

可在低于0°C的环境中稳定储存至少一年。

pictograms

GHS08,GHS05

signalword

Danger

hcodes

H290,H315,H318,H361fd,H373

pcodes

P202 - P234 - P280 - P302 + P352 - P305 + P351 + P338 - P308 + P313

Hazard Classifications

Eye Dam. 1 - Met. Corr. 1 - Repr. 2 - Skin Irrit. 2 - STOT RE 2 Oral

target_organs

Kidney,Liver,Blood

存储类别

12 - Non Combustible Liquids

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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全球贸易项目编号

货号	GTIN
N7653-100ML	04061838256126

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