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N7653

Sigma-Aldrich

对硝基苯磷酸盐液体底物系统

liquid

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别名:
4-硝基苯磷酸二钠 溶液, pNPP
线性分子式:
O2NC6H4OP(O)(ONa)2
CAS号:
4264-83-9
分子量:
263.05
MDL编号:
MFCD00007319
PubChem化学物质编号:
24897835
NACRES:
NA.83

形式

liquid

运输

wet ice

储存温度

−20°C

SMILES string

[Na+].[Na+].[O-][N+](=O)c1ccc(OP([O-])([O-])=O)cc1

InChI

1S/C6H6NO6P.2Na/c8-7(9)5-1-3-6(4-2-5)13-14(10,11)12;;/h1-4H,(H2,10,11,12);;/q;2*+1/p-2

InChI key

VIYFPAMJCJLZKD-UHFFFAOYSA-L

相关类别

一般描述

对硝基苯磷酸盐（pNPP）液体底物系统将对硝基苯磷酸盐、缓冲液和所需的镁阳离子结合在一起，成为一种便捷的即用型单一溶液试剂。pNPP能够产生可溶性终产物，是碱性磷酸酶免疫测定的理想底物。

应用

在碱性磷酸酶测定中，采用p-对硝基苯基磷酸盐液体底物体系作为测定碱性磷酸酶活性的底物。
碱性磷酸酶的底物，能够产生可在405 nm处读取的可溶性黄色终产物。使用3N NaOH可终止反应并在405 nm处读取结果。推荐用于ELISA（微孔）分析，不建议用于膜应用。

生化/生理作用

4-硝基苯基磷酸二钠盐六水(pNPP)是测定蛋白质酪氨酸磷酸酶活性的非蛋白质和非特异性底物。pNPP水解后会生成p-硝基酚。它是碱性磷酸酶常见的显色底物。

注意

可在低于0°C的环境中稳定储存至少一年。

外形

即用型。

象形图

CorrosionHealth hazard
GHS05,GHS08

警示用语：

Danger

危险分类

Eye Dam. 1 - Met. Corr. 1 - Repr. 2 - Skin Irrit. 2 - STOT RE 2 Oral

储存分类代码

8B - Non-combustible, corrosive hazardous materials

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. How should Product N7653, p-Nitrophenyl Phosphate Liquid Substrate System, be used?

    Information on usage is found in our product information sheet (under Documents, above).

  4. What type of application can Product N7653, p-Nitrophenyl Phosphate Liquid Substrate System, be used in?

    This product is recommended for ELISA (microwell) procedures and is not recommended for membrane applications.

  5. What is the concentration of Product N7653, p-Nitrophenyl Phosphate Liquid Substrate System?

    The concentration of this product is considered proprietary.

  6. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  7. What is the Department of Transportation shipping information for this product?

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比色碱性磷酸酶和过氧化物酶底物检测系统

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