N8786
Anti-Nuclear Pore Complex Proteins antibody, Mouse monoclonal
clone 414, purified from hybridoma cell culture
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关于此项目
Conjugate:
unconjugated
Clone:
414, monoclonal
Application:
EM, ICC, IP, WB
Citations:
10
isotype
IgG1
biological source
mouse
conjugate
unconjugated
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
414, monoclonal
form
buffered aqueous solution
mol wt
antigen 62-210 kDa
species reactivity
mouse, rat, yeast, Xenopus, human
concentration
~3 mg/mL
technique(s)
electron microscopy: suitable, immunocytochemistry: suitable, immunoprecipitation (IP): suitable, western blot: 2-4 μg/mL using HeLa total cell extract
shipped in
dry ice
target post-translational modification
unmodified
Quality Level
Gene Information
human ... NPIPB15(440348)
General description
Anti-Nuclear Pore Complex Proteins antibody, Mouse monoclonal (mouse IgG1 isotype) is derived from the hybridoma 414 produced by the fusion of mouse myeloma cells (NS-1 cells) and splenocytes from BALB/c mice immunized with rat liver nuclei extract. The nuclear pore complexes (NPCs) are structurally highly conserved in all eukaryotic cells. They contain 30-40 different proteins resulting in a complex with a molecular weight of ~50 kDa.
Nuclear pore complexes (NPCs) are aqueous channels that impart access to the nucleus and modulate the transport of RNA, proteins and ribosome subunits across the nuclear envelope. Nucleoporins, which are conserved proteins, form a complex network and generate these channels. Each NPC is a large, complex cylindrical structure consisting of around 500 nucleoporins.
Immunogen
rat liver nuclei extract.
Application
Anti-Nuclear Pore Complex Proteins antibody, Mouse monoclonal has been used for immunohistochemistry.
Anti-Nuclear Pore Complex Proteins antibody, Mouse monoclonal has been used in:
- immunoblotting
- immunoprecipitation
- immunocytochemistry
- immunoelectron microscopy
- immunofluorescence
Biochem/physiol Actions
Nucleocytoplasmic exchanges occur through nuclear pore complexes (NPCs) that are circular apertures in the nuclear envelope where the inner and the outer nuclear membrane are joined. Through the NPC there is a passive transport of small molecules (ions and water) that are smaller than 9-10 nm. Large molecules such as RNA and proteins have to be actively transported through the NPC in an energy and signal dependent manner. Many proteins interact with members of the NPC complex.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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存储类别
12 - Non Combustible Liquids
wgk
nwg
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
常规特殊物品
常规特殊物品
低风险生物材料
此项目有
Isolation of the yeast nuclear pore complex.
Rout MP and Blobel G
The Journal of cell biology, 123(4), 771-783 (1993)
EGF signaling and the origin of axial polarity among the insects
Lynch JA, et al.
Current Biology, 20(11), 1042-1047 (2010)
The nuclear pore complex ? structure and function at a glance
Greg Kabachinski
Journal of Cell Science (2015)
Satya Prakash Singh et al.
Molecular biology of the cell, 31(4), 304-317 (2020-01-09)
Simian immunodeficiency virus (SIV) and human immunodeficiency virus 2 (HIV-2) display unique ability to infect nondividing target cells. Viral protein X (Vpx) of HIV-2/SIV is known to be involved in the nuclear import of viral genome in nondividing cells, but
Natalia Wesolowska et al.
eLife, 9 (2020-01-29)
The nucleus of oocytes (germinal vesicle) is unusually large and its nuclear envelope (NE) is densely packed with nuclear pore complexes (NPCs) that are stockpiled for embryonic development. We showed that breakdown of this specialized NE is mediated by an
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Centrifugation separates organelles based on size, shape, and density, facilitating subcellular fractionation across various samples.
亚细胞组分离心分离是一种常用技术，广泛适用于多种类型的细胞和组织。由于不同细胞器的大小、形状和密度不同，因此通过离心，可从温和均质的样品中轻松分离和纯化细胞器组分。
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