N9284
硝基还原酶 来源于大肠杆菌
≥90% (SDS-PAGE), recombinant, expressed in E. coli
别名:
NTRA
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关于此项目
Specific activity:
≥100 units/mL
Assay:
≥90% (SDS-PAGE)
Recombinant:
expressed in E. coli
recombinant
expressed in E. coli
Quality Level
assay
≥90% (SDS-PAGE)
form
lyophilized powder
specific activity
≥100 units/mL
mol wt
monomer 24000
greener alternative product characteristics
Waste Prevention
Safer Solvents and Auxiliaries
Design for Energy Efficiency
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sustainability
Greener Alternative Product
UniProt accession no.
greener alternative category
shipped in
wet ice
storage temp.
−20°C
Gene Information
Escherichia coli K12 ... nfsB(945483)
General description
硝基还原酶是一种黄素蛋白，由NfsB基因编码。它包含一个二聚体，每个亚基具有217个氨基酸和活性位点。该结构具有FMN和与酶结合的底物。
含有PBS的冻干粉。 作为辅药不含有BSA
Application
大肠杆菌来源硝基还原酶可结合猪肝酯酶(PLE)用于偶联结合反应。它还还可结合探针HyCL-3和HyCL-4-AM针对大鼠肝微粒体进行化学发光响应研究。
Biochem/physiol Actions
硝基还原酶(NTR)可催化还原硝基芳香族底物和醌。NTR的F124K突变体可用于癌症治疗，改善药物CB1954的敏化作用。
硝基还原酶增强了生命体对于含氮药物，比如甲硝唑的敏感度，它将氮基团转化为有毒的氮自由基。
硝基还原酶在NADPH或NADH为还原剂的氧还系统中有重要作用。
能够还原奎宁。 该酶可以激活抗体引导酶前药治疗的前药。
Preparation Note
使用没有动物组分的材料生产。
Other Notes
在pH7.4、37℃、存在甲萘醌和NADH的条件下，一单位酶每分钟能够还原1μmole细胞色素C。
存储类别
13 - Non Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
常规特殊物品
此项目有
G M Anlezark et al.
Biochemical pharmacology, 50(5), 609-618 (1995-08-25)
A nitroreductase isolated and purified from Escherichia coli B has been demonstrated to have potential applications in ADEPT (antibody-directed enzyme prodrug therapy) by its ability in vitro to reduce dinitrobenzamides (e.g. 5-aziridinyl 2,4-dinitrobenzamide, CB 1954 and its bischloroethylamino analogue, SN
Sébastien Jenni et al.
Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy, 248, 119179-119179 (2020-11-30)
The ever-growing demand for fluorogenic dyes usable in the rapid construction of analyte-responsive fluorescent probes, has recently contributed to a revival of interest in the chemistry of diketopyrrolopyrrole (DPP) pigments. In this context, we have explored the potential of symmetrical
Generation of Escherichia coli nitroreductase mutants conferring improved cell sensitization to the prodrug CB1954
Grove JI, et al.
Cancer research, 63(17), 5532-5537 (2003)
全球贸易项目编号
| 货号 | GTIN |
|---|---|
| N9284-1MG | 04061834118787 |