N9876
4-硝基苯基丁酸酯
lipase substrate, chromogenic, esterase substrate, ≥98%, liquid
别名:
p-硝基苯基丁酸酯, 丁酸4-硝基苯酯
一般描述
4-硝基苯基丁酸酯是酯酶 和脂肪酶的底物。这些酶水解4-硝基苯基丁酸酯可释放发色团4-硝基苯酚，其可在415 nm下通过分光光度法进行分析。
应用
4-硝基苯基丁酸酯被用作：
- 源自单核细胞的巨噬细胞系对聚乙烯反应过程中酯酶活性的底物
- 分枝杆菌磷脂酶A和分枝杆菌角质酶活性的底物
- 测定缓冲液和溶剂对人羧酸酯酶的影响的底物
警示用语：
Warning
危险声明
危险分类
Skin Sens. 1
储存分类代码
10 - Combustible liquids
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter
历史批次信息供参考:
分析证书(COA)
Lot/Batch Number
Nathalia S Rios et al.
Biochimica et biophysica acta. Proteins and proteomics, 1867(9), 741-747 (2019-06-16)
The lipase from Pseudomonas fluorescens (PFL) has been immobilized on glyoxyl-octyl agarose and compared to the enzyme immobilized on octyl-agarose. Thus, PFL was immobilized at pH 7 on glyoxyl-octyl support via lipase interfacial activation and later incubated at pH 10.5 for 20 h
Differential response to chemically altered polyethylene by activated mature human monocyte-derived macrophages
Xing S, et al.
Biomaterials, 23(17), 3595-3602 (2002)
Li-Chun Wang et al.
Journal of food and drug analysis, 26(3), 973-984 (2018-07-07)
Obesity is associated with higher risks of developing diabetes and cardiovascular disease. Green tea, rich in polyphenolic compounds such as epigallocatechin gallate (EGCG) and epigallocatechin (EGC), has been shown to display anti-obesity effects. Houttuynia cordata leaves have also been shown
Jose J Virgen-Ortíz et al.
Enzyme and microbial technology, 96, 30-35 (2016-11-23)
Lipase B from Candida antarctica (CALB) has been physically immobilized on octyl-agarose via interfacial activation. The incubation of the enzyme in 80% ethanol at pH 5 and 25°C has not significant effect on enzyme activity. Moreover, the hydrolysis of 100mM
Stephane Emond et al.
Nature communications, 11(1), 3469-3469 (2020-07-12)
Insertions and deletions (InDels) are frequently observed in natural protein evolution, yet their potential remains untapped in laboratory evolution. Here we introduce a transposon-based mutagenesis approach (TRIAD) to generate libraries of random variants with short in-frame InDels, and screen TRIAD
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