用途
sufficient for 25 purifications
质量水平
环保替代产品特性
Designing Safer Chemicals
Learn more about the Principles of Green Chemistry.
sustainability
Greener Alternative Product
技术
DNA purification: suitable
环保替代产品分类
, Aligned
储存温度
15-25°C
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一般描述
GenElute HP质粒中量制备试剂盒可以超快速、高效的从重组大肠杆菌培养物大批量制备质粒。该试剂盒结合了硅胶膜技术和离心或真空形式的便利性。该试剂盒还附带专用的滤器卡夹，从而替代了碱裂解之后的离心分离步骤。裂解之后，DNA结合到硅胶膜上，并通过一次简单的洗涤步骤去除了污染物。随后在水或TE缓冲液中，通过离心处理洗脱DNA。
纯化所得的质粒DNA适于包括限制性酶切和测序在内的一系列分子生物学应用。
纯化所得的质粒DNA适于包括限制性酶切和测序在内的一系列分子生物学应用。
我们致力于为您提供更环保的替代产品，以符合“绿色化学的12项原则”的一项或多项原则要求。相比标准DNA提取方法使用苯酚和氯仿，该产品是一种更安全的化学产品。
应用
GenElute™ HP 质粒Midiprep试剂盒已用于分离和纯化质粒。
特点和优势
- 可在30分钟内从收集所得的细菌培养物中纯化出质粒DNA
- 可纯化出多达350 μg高拷贝数质粒DNA
- 真空或离心形式可灵活选择
- 无需进行苯酚/氯仿萃取或乙醇沉淀步骤
- 所含的塑料成分更少，减少了废物量
包装
<strong>Components included:</strong><UL><LI><strong>Qty in kit:</strong>2<strong> Part number: </strong>C4228<strong> Component description: </strong>Collection Tubes, 15 ml conical</LI><LI><strong>Qty in kit:</strong>1<strong> Part number: </strong>R1149<strong> Component description: </strong>Resuspension Solution</LI><LI><strong>Qty in kit:</strong>1<strong> Part number: </strong>N1285<strong> Component description: </strong>Neutralization Solution</LI><LI><strong>Qty in kit:</strong>1<strong> Part number: </strong>B4683<strong> Component description: </strong>Binding Solution</LI><LI><strong>Qty in kit:</strong>1<strong> Part number: </strong>G4792<strong> Component description: </strong>[TM="GenElute} HP Midiprep Binding Columns</LI><LI><strong>Qty in kit:</strong>1<strong> Part number: </strong>E7777<strong> Component description: </strong>Elution Solution (10 mM Tris-HCl, pH 8.5)</LI><LI><strong>Qty in kit:</strong>1<strong> Part number: </strong>W0263<strong> Component description: </strong>Wash Solution 1</LI><LI><strong>Qty in kit:</strong>1<strong> Part number: </strong>W4639<strong> Component description: </strong>Wash Solution 2</LI><LI><strong>Qty in kit:</strong>1<strong> Part number: </strong>R6148<strong> Component description: </strong>RNase A solution</LI><LI><strong>Qty in kit:</strong>1<strong> Part number: </strong>C2112<strong> Component description: </strong>Column Preparation Solution</LI><LI><strong>Qty in kit:</strong>1<strong> Part number: </strong>G8917<strong> Component description: </strong>[TM="GenElute} HP Midiprep Filter Syringes</LI><LI><strong>Qty in kit:</strong>1<strong> Part number: </strong>L1912<strong> Component description: </strong>Lysis Solution</LI></UL>
其他说明
更多信息，请见www.sigma-aldrich.com/genelutehp。
法律信息
GenElute is a trademark of Sigma-Aldrich Co. LLC
警示用语：
Danger
危险分类
Acute Tox. 4 Oral - Eye Dam. 1 - Flam. Liq. 3 - Met. Corr. 1 - Resp. Sens. 1 - Skin Corr. 1B - STOT SE 3
靶器官
Central nervous system
储存分类代码
3 - Flammable liquids
法规信息
危险化学品
低风险生物材料
此项目有
历史批次信息供参考:
分析证书(COA)
Lot/Batch Number
Vanessa E Gray et al.
G3 (Bethesda, Md.), 9(11), 3683-3689 (2019-09-29)
Despite the importance of Aβ aggregation in Alzheimer's disease etiology, our understanding of the sequence determinants of aggregation is sparse and largely derived from in vitro studies. For example, in vitro proline and alanine scanning mutagenesis of Aβ40 proposed core
Genome-wide CRISPR screens reveal a Wnt-FZD5 signaling circuit as a druggable vulnerability of RNF43-mutant pancreatic tumors.
Steinhart Z
Nature Medicine, 23(1), 60-68 (2017)
The distal upstream promoter in Ly49 genes, Pro1, is active in mature NK cells and T cells, does not require TATA boxes, and displays enhancer activity.
Gays F
Journal of Immunology, 194(12), 6068-6081 (2015)
Stephanie P Cartwright et al.
BMC research notes, 10(1), 340-340 (2017-07-29)
Patients with dual hepatitis B (HBV) and hepatitis D (HDV) virus infection are at an increased risk of progression to liver cirrhosis and hepatocellular carcinoma than patients with a single viral infection. Treatment of viral hepatitis due to dual HBV/HDV
Melissa A Chiasson et al.
eLife, 9 (2020-09-02)
Vitamin K epoxide reductase (VKOR) drives the vitamin K cycle, activating vitamin K-dependent blood clotting factors. VKOR is also the target of the widely used anticoagulant drug, warfarin. Despite VKOR's pivotal role in coagulation, its structure and active site remain
实验方案
使用CRISPR/Cas9系统生成正确的转基因小鼠需要充分理解正在测试的假设。了解如何创建成功的小鼠模型项目。
相关内容
DNA和RNA提取是分子生物学中的一种基本方法。高质量、高纯度的核酸在一系列研究和临床应用中发挥着重要的作用。因此，核酸纯化是分子生物学和基因组工作流程中的第一步。
Overview of common techniques and downstream applications for extraction and purification of genomic DNA, plasmid DNA, and total RNA from cells, tissue, blood, viruses, and other sample types.
Tools for plasmid preparation, in vitro transcription, mRNA purification, and LNP formulation in mRNA-based vaccine and therapeutic development.
用于质粒制备、体外转录、mRNA 纯化和 LNP 制剂的工具，用于基于 mRNA 的疫苗和疗法开发。
我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
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