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Merck
CN

NA0410

GenElute™ HP 无内毒素质粒大提试剂盒

greener alternative

sufficient for 25 preparations

别名:

Gen Elute, GenElute 无内毒素质粒试剂盒

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NACRES:
NA.52
UNSPSC Code:
41105501

主要文件

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产品名称

GenElute™ HP 无内毒素质粒大提试剂盒, sufficient for 25 preparations

usage

sufficient for 25 preparations

greener alternative product characteristics

Designing Safer Chemicals
Learn more about the Principles of Green Chemistry.

sustainability

Greener Alternative Product

technique(s)

DNA extraction: suitable

greener alternative category

Aligned,

storage temp.

15-25°C

Quality Level

200

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General description

Click here to view the kit protocol
GenElute HP 无内毒素质粒大量制备试剂盒可以简单、快速地从重组大肠杆菌培养物中分离不含内毒素的质粒DNA。该试剂盒采用了可快速澄清细菌裂解液的滤柱和可捕获质粒DNA的硅胶柱。配套的专利溶液可使质粒DNA结合到结合柱上，同时避免内毒素随之吸附。该技术使得用户可以维持一致的、低于 0.1 EU/μg 质粒DNA的内毒素水平。

制备所得的优质、不含内毒素DNA可直接供包括内毒素敏感细胞转染等最严格的应用使用。

Biochem/physiol Actions

通过离心分离法收集过夜的重组大肠杆菌培养液，然后通过改性SDS 碱裂解法进行处理。过滤澄清裂解液，然后加入针对无内毒素质粒制备而优化的结合溶液。随后，质粒捕获到硅胶膜上，同时避免内毒素随之吸附到膜上。通过两遍洗涤步骤，去除污染物。最后，在不含内毒素的水中洗脱结合所得的DNA。回收的质粒DNA主要以超螺旋形式存在。所含的基因组DNA和RNA均低于溴化丙啶染色琼脂糖凝胶电泳的检测水平。

Application

GenElute™ HP Endotoxin-Free Plasmid Maxiprep试剂盒已被用于分离猪肝和大肠杆菌感受态细胞中的质粒。

Features and Benefits

  • 可纯化出多达1.2 mg内毒素水平≤0.1 EU/μg高拷贝数质粒DNA
  • 从沉淀细胞到获得纯化的质粒，仅需35分钟
  • 方便的真空形式

Other Notes

更多信息，请见www.sigma-aldrich.com/genelutehp。

Legal Information

GenElute is a trademark of Sigma-Aldrich Co. LLC

signalword

Danger

Hazard Classifications

Acute Tox. 4 Oral - Eye Irrit. 2 - Flam. Liq. 3 - Met. Corr. 1 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

target_organs

Central nervous system

存储类别

3 - Flammable liquids

flash_point_f

77.0 °F - closed cup

flash_point_c

25 °C - closed cup

法规信息

低风险生物材料
危险化学品
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历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
0000476571
0000476571
0000420536
0000420536
0000380779

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相关内容

Endotoxin-free Plasmid DNA Purification

GenElute HP Plasmid Maxiprep Kit isolates endotoxin-free plasmid DNA in 35 minutes, meeting industry standards.

mRNA Synthesis

Tools for plasmid preparation, in vitro transcription, mRNA purification, and LNP formulation in mRNA-based vaccine and therapeutic development.

mRNA 合成

用于质粒制备、体外转录、mRNA 纯化和 LNP 制剂的工具，用于基于 mRNA 的疫苗和疗法开发。

GenElute HP Endotoxin-Free Plasmid Maxiprep Kit
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