一般描述
应用
特点和优势
- 可在8分钟内纯化出多达100 μl或10 μg 的PCR扩增产物DNA。
- 可回收95%长度介于100 bp至10 kb之间的PCR产物
- 可去除99%以上的引物和其他成分
- 不需要去除有机萃取引入的矿物油
- 相对于其他市场领先竞争对手，可多获得40%的纯化制备物
其他说明
原理
法律信息
警示用语：
Warning
危险分类
Acute Tox. 4 Oral - Eye Irrit. 2 - Met. Corr. 1 - Skin Irrit. 2 - STOT SE 3
靶器官
Central nervous system
储存分类代码
8A - Combustible, corrosive hazardous materials
WGK
WGK 2
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
分析证书(COA)
输入产品批号来搜索 分析证书(COA) 。批号可以在产品标签上"批“ （Lot或Batch）字后找到。
已有该产品？
为方便起见，与您过往购买产品相关的文件已保存在文档库中。
难以找到您所需的产品或批次号码？
如何查找产品货号
在网站页面上，产品编号会附带包装尺寸/数量一起显示（例如：T1503-25G）。请确保 在“产品编号”字段中仅输入产品编号 (示例: T1503).
示例
其它示例：
705578-5MG-PW
PL860-CGA/SHF-1EA
MMYOMAG-74K-13
1000309185
输入内容 1.000309185)
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如何查找COA批号
批号可以在产品标签上"批“ （Lot或Batch）字后面找到。
Aldrich 产品
如果您查询到的批号为 TO09019TO 等，请输入去除前两位字母的批号：09019TO。
如果您查询到的批号含有填充代码（例如05427ES-021），请输入去除填充代码-021的批号：05427ES。
如果您查询到的批号含有填充代码（例如 STBB0728K9），请输入去除填充代码K9的批号：STBB0728。
未找到您寻找的产品？
部分情况下，可能未在线提供COA。如果搜索不到COA，可在线索取。
Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.
How do I get lot-specific information or a Certificate of Analysis?
The lot specific COA document can be found by entering the lot number above under the "Documents" section.
How do I find price and availability?
There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote. USA customers: 1-800-325-3010 or view local office numbers.
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
Can Product NA1020, GenElute™ PCR Clean-Up Kit, be used for isolation of PCR products labeled with digoxigenin?
Although we have not tested the kit with digoxigenin-labeled PCR products specifically, it should be fine to use
Can Product No. NA1020, GenElute™ PCR Clean-Up Kit, be used to clean up PCR products larger than 10 kb?
We haven't tested anything bigger than 10 kb, but we would expect larger DNA to bind well. That said, the bound DNA might be more difficult to elute. Heating the elution solution to 60-65°C may help with elution. Incubating a few minutes to allow the elution solution to soak into the binding matrix may also help.
When using Product No. NA1020, GenElute™ PCR Clean-Up Kit, what is the largest amount of PCR reaction sample that can be processed on one column?
The upper limit on the PCR reaction volume that can be cleaned up is 100 μl. In that scenario, 500 μl of binding solution would be added for a total of 600 μl volume being applied to the column.
When using GenElute™ PCR Clean-Up Kit, Product No. NA1020, Can the amount of elution buffer be reduced in order to obtain a more concentrated eluate?
One may indeed increase concentration by reducing elution volume, but we would not suggest going lower than 25-30 μl. One must keep in mind that if a volume of less than 50 μl is used (for example, 30 μl), the concentration will increase, but the total yield will also be reduced.
Can GenElute™ PCR Clean-Up Kit, Product NA1020, be used for isolation of labeled PCR products?
This kit will work with labeled PCR products and no modifications to the standard protocol are needed.
My question is not addressed here, how can I contact Technical Service for assistance?
Ask a Scientist here.
实验方案
Follow this procedure to rapidly purify single-stranded or double-stranded PCR amplification products (100 bp to 10 kb) from excess primers, nucleotides, DNA polymerase, oil and salts using the GenElute™ PCR Clean-up Kit.
The SeqPlex DNA Amplification Kit for whole genome amplification (WGA) is designed to facilitate next-generation sequencing (NGS) from extremely small quantities or from degraded/highly fragmented DNA
Genomic DNA from soil samples can be easily damaged by nucleases and contaminating debris resulting in low DNA yields. As a result, the researcher’s ability to perform downstream analysis may be compromised. After isolating DNA from the soil sample, the GenomePlex® Whole Genome Amplification Protocol is followed
面向全基因组扩增(WGA)的SeqPlexDNA扩增试剂盒(SEQXE)专用于对极小量或降解/高度片段化的DNA进行下一代测序(NGS)。
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