General description
草酰乙酸脱羧酶 (OAD) 在厌氧菌中起 Na 泵的作用。是一种由 α、β 和 γ 三个亚基组成的膜蛋白，α 亚基含有羧化酶活性。
Application
来自假单胞菌 属的草酰乙酸脱羧酶已被用于消化低分子量（LMW）人乳馏分（5kF馏分）并可用作测定嗜热纤维梭状芽孢杆菌（C. thermocellum）草酰乙酸脱羧酶活性的阳性对照。
草酰乙酸脱羧酶已用于一项研究，以评估 Na (+)-谷氨酸盐转运蛋白 GltS 的转换率和折返环的可及性。 在一项研究中也被用来研究木葡糖酸醋杆菌对不同碳源的发酵和代谢特性。
Biochem/physiol Actions
草酰乙酸脱羧酶催化草酰乙酸盐的脱羧反应，其活性不需要锰和镁。它与多种革兰氏阴性菌有关。
Other Notes
在 25℃、pH 8.0 的条件下，每分钟有 1 个单位的草酰乙酸转化为丙酮酸和 CO2
存储类别
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
法规信息
常规特殊物品
此项目有
N E Labrou et al.
Archives of biochemistry and biophysics, 365(1), 17-24 (1999-05-01)
Oxaloacetate decarboxylase (OXAD), the enzyme that catalyzes the decarboxylation of oxaloacetate to pyruvic acid and carbon dioxide, was purified 245-fold to homogeneity from Pseudomonas stutzeri. The three-step purification procedure comprised anion-exchange chromatography, metal-chelate affinity chromatography, and biomimetic-dye affinity chromatography. Estimates
Determining citrate in fruit juices using a biosensor with citrate lyase and oxaloacetate decarboxylase in a flow injection analysis system.
Kim, M.
Food Chemistry, 99(4), 851-857 (2006)
Pablo D Sender et al.
FEBS letters, 570(1-3), 217-222 (2004-07-15)
The citM gene from Lactococcus lactis CRL264 was demonstrated to encode for an oxaloacetate decarboxylase. The enzyme exhibits high levels of similarity to malic enzymes (MEs) from other organisms. CitM was expressed in Escherichia coli, purified and its oxaloacetate decarboxylase
全球贸易项目编号
| 货号 | GTIN |
|---|---|
| O4878-1KU | 04061833631164 |
| O4878-500UN | 04061833631171 |