产品名称
OPI 培养基添加剂, Hybri-Max™, γ-irradiated, powder, suitable for hybridoma
grade
Hybri-Max™
sterility
γ-irradiated
form
powder
concentration
18-40 mg/vial
technique(s)
cell culture | hybridoma: suitable
impurities
endotoxin, tested
storage temp.
−20°C
Quality Level
Application
OPI培养基添加剂已用于转基因小鼠(XenoMouse)品系和BALB/c雌鼠来源的杂交瘤细胞系生长。
Other Notes
配方含有：0.15g草酰乙酸，0.05g丙酮酸盐和0.0082g胰岛素。
Preparation Note
10mL 无菌水可复原一小瓶本品。初次复原时请勿使用细胞培养基。每小瓶足以制备 1 升培养基。最终工作浓度：1mM 草酰乙酸盐、0.45mM 丙酮酸盐、0.2U/ml 胰岛素。
Legal Information
Hybri-Max is a trademark of Sigma-Aldrich Co. LLC
signalword
Danger
hcodes
Hazard Classifications
Eye Dam. 1 - Skin Corr. 1 - Skin Sens. 1
存储类别
8A - Combustible corrosive hazardous materials
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
常规特殊物品
此项目有
Gabriela A Canziani et al.
Analytical biochemistry, 325(2), 301-307 (2004-01-31)
Experimental and data analysis protocols were developed to screen antibodies from hybridoma culture supernatants using Biacore surface plasmon resonance biosensor platforms. The screening methods involved capturing antibodies from crude supernatants using Fc-specific antibody surfaces and monitoring antigen binding at a
Andrew W Drake et al.
Analytical biochemistry, 328(1), 35-43 (2004-04-15)
Two biophysical methods, Biacore and KinExA, were used to kinetically and thermodynamically characterize high-affinity antigen/antibody complexes. Three to five independent experiments were performed on each platform with three different antigen/antibody complexes possessing nanomolar to picomolar equilibrium dissociation constants. By monitoring
Jun Yang et al.
Clinical biochemistry, 48(13-14), 843-848 (2015-05-31)
Neurogranin (NRGN) is a small neuronal protein that plays an important role in synaptic signaling by regulating calmodulin (CaM) availability. In this study, we developed an ELISA to measure NRGN quantitatively in serum samples from a cohort of acute traumatic
Andrew W Drake et al.
Analytical biochemistry, 328(1), 35-43 (2004-04-15)
Two biophysical methods, Biacore and KinExA, were used to kinetically and thermodynamically characterize high-affinity antigen/antibody complexes. Three to five independent experiments were performed on each platform with three different antigen/antibody complexes possessing nanomolar to picomolar equilibrium dissociation constants. By monitoring
Tzu-Ling Sung et al.
PLoS pathogens, 5(1), e1000263-e1000263 (2009-01-17)
Cyclin T1 is a regulatory subunit of a general RNA polymerase II elongation factor known as P-TEFb. Cyclin T1 is also required for Tat transactivation of HIV-1 LTR-directed gene expression. Translation of Cyclin T1 mRNA has been shown to be
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