标签
FLAG® tagged
表单
buffered aqueous solution
分子量
size 6174 bp
菌种筛选
kanamycin
哺乳动物细胞筛选
puromycin
复制起点
pUC (500 copies)
肽切割
no cleavage
肽标签位置
C-terminal
启动子
Promoter name: CMV
Promoter activity: constitutive
Promoter type: mammalian
报告基因
none
运输
ambient
储存温度
−20°C
一般描述
This plasmid is designed to express tagged proteins in mammalian cells either by transient transfection or by creating stable cell lines. It contains a puromycin resistance expression cassette using the human Ubiquitin promoter to drive expression and allow for the selection of cells containing the plasmid.
About the Peptide Tag:This plasmid contains a c-terminal FLAG epitope tag that can be fused to a gene of interest to allow protein detection and/or purification. The sequence of the tag is: DYKDDDDK.
About the Cleavage Tag:This plasmid does not contain a protease cleavage site.
Promoter Expression Level: This plasmid contains the mammalian CMV promoter to drive gene expression. We have tested all of our mammalian promoters in a range of cell types and CMV is consistently the strongest in those we have studied. However there are many reports of the CMV promoter demonstrating silencing by methylation in long-term culture.
About the Peptide Tag:This plasmid contains a c-terminal FLAG epitope tag that can be fused to a gene of interest to allow protein detection and/or purification. The sequence of the tag is: DYKDDDDK.
About the Cleavage Tag:This plasmid does not contain a protease cleavage site.
Promoter Expression Level: This plasmid contains the mammalian CMV promoter to drive gene expression. We have tested all of our mammalian promoters in a range of cell types and CMV is consistently the strongest in those we have studied. However there are many reports of the CMV promoter demonstrating silencing by methylation in long-term culture.
序列
To view sequence information for this product, please visit the product page
分析说明
To view the Certificate of Analysis for this product, please visit www.oxgene.com
法律信息
FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
储存分类代码
12 - Non Combustible Liquids
闪点(°F)
Not applicable
闪点(°C)
Not applicable
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