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OGS3425

Sigma-Aldrich

PSF-CMV-PURO-COOH-HA - C-TERMINAL HA TAG MAMMALIAN PLASMID

plasmid vector for molecular cloning

别名:

cloning vector, expression vector, molecular cloning vector, plasmid, plasmid vector, snapfast vector, vector

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1 EA
¥2,733.59
5 EA
¥10,387.65
10 EA
¥19,135.14

¥2,733.59


预计发货时间2025年10月20日详情


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1 EA
¥2,733.59
5 EA
¥10,387.65
10 EA
¥19,135.14

About This Item

UNSPSC代码:
12352200
NACRES:
NA.85

¥2,733.59


预计发货时间2025年10月20日详情


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标签

Hemagglutinin A (HA) tagged

表单

buffered aqueous solution

分子量

size 6177 bp

菌种筛选

kanamycin

哺乳动物细胞筛选

puromycin

复制起点

pUC (500 copies)

肽切割

no cleavage

肽标签位置

C-terminal

启动子

Promoter name: CMV
Promoter activity: constitutive
Promoter type: mammalian

报告基因

none

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GF12226632GF11124863GF00758347
form

foil

form

foil

form

foil

form

foil

manufacturer/tradename

Goodfellow 022-251-02

manufacturer/tradename

Goodfellow 122-266-32

manufacturer/tradename

Goodfellow 111-248-63

manufacturer/tradename

Goodfellow 007-583-47

size

25 × 25 mm

size

50 × 50 mm

size

-

size

-

thickness

3.0 mm

thickness

3.0 mm

thickness

0.5 mm

thickness

0.5 mm

一般描述

This plasmid is designed to express tagged proteins in mammalian cells either by transient transfection or by creating stable cell lines. It contains a puromycin resistance expression cassette using the human Ubiquitin promoter to drive expression and allow for the selection of cells containing the plasmid.

About the Peptide Tag:This plasmid contains a c-terminal Influenza Hemagglutinin (HA) epitope tag that can be fused to a gene of interest to allow protein detection and/or purification. The sequence of the tag is: YPYDVPDYA.

About the Cleavage Tag:This plasmid does not contain a protease cleavage site.

Promoter Expression Level: This plasmid contains the mammalian CMV promoter to drive gene expression. We have tested all of our mammalian promoters in a range of cell types and CMV is consistently the strongest in those we have studied. However there are many reports of the CMV promoter demonstrating silencing by methylation in long-term culture.

分析说明

To view the Certificate of Analysis for this product, please visit www.oxgene.com

其他说明

To view sequence information for this product, please visit the product page

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说明
价格

储存分类代码

12 - Non Combustible Liquids

闪点(°F)

Not applicable

闪点(°C)

Not applicable

法规信息

新产品

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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