OGS589
PBR322 - PBR322 低拷贝克隆载体
plasmid vector for molecular cloning
别名:
snapfast 载体
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关于此项目
NACRES:
NA.85
UNSPSC Code:
12352200
Origin of replication:
BR322 (15 copies)
Bacteria selection:
ampicillin
Reporter gene:
none
Peptide cleavage:
no cleavage
form
buffered aqueous solution
mol wt
size 4361 bp
bacteria selection
ampicillin
origin of replication
BR322 (15 copies)
peptide cleavage
no cleavage
reporter gene
none
shipped in
ambient
storage temp.
−20°C
General description
该质粒含有 BR322（低拷贝数）复制起点，并为每个细菌细胞提供约 20 个质粒拷贝。它还包含用于选择转染哺乳动物细胞的MCS和Kan 抗性的哺乳动物CMV启动子上游。低拷贝数质粒对非常大的转基因或对细菌细胞有毒性的转基因是有用的。
启动子表达水平：
启动子表达水平：
Application
BR322 信息： BR322 是从大肠杆菌中分离得到的野生型质粒。其含有低拷贝来源，允许质粒以每个细胞约 10-100 个质粒拷贝的频率维持。通常每个细胞的这一数字接近 30-40个拷贝。最常见的克隆质粒含有该质粒中称为pUC的复制起点的衍生物。pUC 的起源是通过去除 Rep 阻遏蛋白形成的。阻遏蛋白通常在大肠杆菌中调节质粒拷贝数，也在复制起始点本身进行单点突变。点突变是复制起点之间最重要的差异。该质粒可作为通用低拷贝克隆载体。
Analysis Note
欲查看本品的分析证书，请访问 www.oxgene.com
Other Notes
欲查看本品的序列信息，请访问 产品页面。
存储类别
12 - Non Combustible Liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
新产品
此项目有
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