OGS64
PSF-CMV-COOH-P2A-2 - FMDV P2A PLASMID
plasmid vector for molecular cloning
别名:
cloning vector, expression vector, molecular cloning vector, plasmid, plasmid vector, snapfast vector, vector
标签
P2A tagged
表单
buffered aqueous solution
分子量
size 4279 bp
菌种筛选
kanamycin
复制起点
pUC (500 copies)
肽切割
no cleavage
启动子
Promoter name: CMV
Promoter activity: constitutive
Promoter type: mammalian
报告基因
none
运输
ambient
储存温度
−20°C
一般描述
This vector adds a Foot and Mouth Disease P2A peptide between the primary MCS (NotI-XbaI but XbaI is ablated in this vector) and the fusion MCS (ClaI-NheI). The P2A peptide coding sequence is: GSGATNFSLLKQAGDVEENPGP. The peptide self-cleaves between the penultimate glycine residue and the final proline residue. This leaves the first (upstream) protein in the polypeptide with the majority of the amino acids of the P2A peptide on the C-terminus whilst the last (downstream) protein has a proline remaining on the N-terminus. We have not confirmed if secretory proteins are able to detach from cytosolic proteins when fused using this method. The intracellular trafficking of your proteins should therefore be considered.
Promoter Expression Level: This plasmid contains the mammalian CMV promoter to drive gene expression. We have tested all of our mammalian promoters in a range of cell types and CMV is consistently the strongest in those we have studied. However there are many reports of the CMV promoter demonstrating silencing by methylation in long-term culture.
Promoter Expression Level: This plasmid contains the mammalian CMV promoter to drive gene expression. We have tested all of our mammalian promoters in a range of cell types and CMV is consistently the strongest in those we have studied. However there are many reports of the CMV promoter demonstrating silencing by methylation in long-term culture.
应用
This vector is designed to allow two genes to be inserted one on either site of the P2A self-cleaving tag. This can be achieved using standard cloning methodologies.
Multiple Cloning Site Notes: There is a start codon in the NcoI site can be removed by digestion with KpnI if required. The MCS for gene insertions in most SnapFast vectors extends from NotI to XbaI however in this vector the XbaI site has been ablated to remove the stop codon in the site and allow read-through into the P2A coding sequence.
Multiple Cloning Site Notes: There is a start codon in the NcoI site can be removed by digestion with KpnI if required. The MCS for gene insertions in most SnapFast vectors extends from NotI to XbaI however in this vector the XbaI site has been ablated to remove the stop codon in the site and allow read-through into the P2A coding sequence.
分析说明
To view the Certificate of Analysis for this product, please visit www.oxgene.com
其他说明
To view sequence information for this product, please visit the product page
储存分类代码
12 - Non Combustible Liquids
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
新产品
此项目有
Diana Romero et al.
Carcinogenesis, 37(1), 18-29 (2015-10-28)
Dickkopf-3 (Dkk-3) is a secreted protein whose expression is downregulated in many types of cancer. Endogenous Dkk-3 is required for formation of acini in 3D cultures of prostate epithelial cells, where it inhibits transforming growth factor (TGF)-β/Smad signaling. Here, we
Geoffrey M Lynn et al.
Nature biotechnology, 33(11), 1201-1210 (2015-10-27)
The efficacy of vaccine adjuvants such as Toll-like receptor agonists (TLRa) can be improved through formulation and delivery approaches. Here, we attached small molecule TLR-7/8a to polymer scaffolds (polymer-TLR-7/8a) and evaluated how different physicochemical properties of the TLR-7/8a and polymer
Alexander C Cerny et al.
PLoS genetics, 11(10), e1005578-e1005578 (2015-10-29)
Recycling of signaling proteins is a common phenomenon in diverse signaling pathways. In photoreceptors of Drosophila, light absorption by rhodopsin triggers a phospholipase Cβ-mediated opening of the ion channels transient receptor potential (TRP) and TRP-like (TRPL) and generates the visual
Jin-Gyoung Jung et al.
PLoS genetics, 10(10), e1004751-e1004751 (2014-10-31)
The Notch3 signaling pathway is thought to play a critical role in cancer development, as evidenced by the Notch3 amplification and rearrangement observed in human cancers. However, the molecular mechanism by which Notch3 signaling contributes to tumorigenesis is largely unknown.
我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
联系客户支持