P0056
Anti-POU5F1 (Oct4) antibody produced in rabbit
~1.0 mg/mL, affinity isolated antibody, buffered aqueous solution
别名:
Anti-OCT3, Anti-OCT4, Anti-OTF3, Anti-OTF4, Anti-Octamer-binding transcription factor 3, Anti-Pou domain, Class 5, Transcription factor 1
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关于此项目
NACRES:
NA.41
UNSPSC Code:
12352203
Conjugate:
unconjugated
Clone:
polyclonal
Application:
IF, IP, WB
Citations:
11
biological source
rabbit
conjugate
unconjugated
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
form
buffered aqueous solution
mol wt
antigen ~43 kDa
species reactivity
human
concentration
~1.0 mg/mL
technique(s)
immunoprecipitation (IP): 5-10 μg using NT2 cell lysate, indirect immunofluorescence: 2-5 μg/mL using paraformaldhyde-fixed F9 cells, western blot: 1-2 μg/mL using NT2 cell lysate
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... POU5F1(5460)
mouse ... Pou5f1(18999)
rat ... Pou5f1(294562)
General description
POU class 5 homeobox 1 (POU5F1) recognizes defined octamer DNA element by a shared, conserved DNA-binding domain, namely the POU domain.
POU class 5 homeobox 1 (POU5F1), also known as octamer-binding transcription factor-4 (OCT-4), is expressed in embryonic stem cells and germ cells. It belongs to the POU family of transcription factors. Three splice variants of the protein (OCT4A, OCT4B and OCT4B1) have been identified. The gene encoding this transcription factor is localized on human chromosome 6p21.33.
Immunogen
synthetic peptide corresponding to amino acids 19-34 of human POU5F1, conjugated to KLH. The corresponding sequence in rat is 81% homologous.
Application
Anti-POU5F1 (Oct4) antibody produced in rabbit has been used in Western Blotting.
Biochem/physiol Actions
POU class 5 homeobox 1 (POU5F1) may be a tumor initiating stem-like cell (TSC)-related marker. It has a role in stem cell pluripotency and self-renewal, and also modulates differentiation. The protein has been shown to be expressed in several cancers. POU5F1 is also vital for the induced pluripotent stem cell (iPSC) process.
POU5F1 or Oct4/Oct3 (Octamer-binding transcription factor), functions as an early developmental control gene. It has been recognized as fundamental in the maintenance of pluripotency in embryonic stem cells and in primordial germ cells. It is down regulated in all differentiated somatic cell types both in vivo and in vitro. This is being accomplished through increased DNA methylation and structural changes involving the immediate upstream regulatory region. POU5F1 serves as a marker for germ cell tumors (GCTs) eliciting pluripotentiality, especially including embryonal, seminoma/ dysgerminoma/ germinoma.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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存储类别
10 - Combustible liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
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