P0082
Monoclonal Anti-POU5F1 (Oct4) antibody produced in mouse
~1.0 mg/mL, clone PF-29, purified immunoglobulin, buffered aqueous solution
别名:
Anti-OCT3, Anti-OCT4, Anti-OTF3, Anti-POU domain, class 5, transcription factor 1
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关于此项目
NACRES:
NA.41
UNSPSC Code:
12352203
Conjugate:
unconjugated
Clone:
PF-29, monoclonal
Application:
immunocytochemistry
indirect ELISA
western blot
indirect ELISA
western blot
Species reactivity:
human
Citations:
7
Technique(s):
immunocytochemistry: suitable
indirect ELISA: suitable
western blot: 0.5-1 μg/mL using NT2/D1 total cell extract
indirect ELISA: suitable
western blot: 0.5-1 μg/mL using NT2/D1 total cell extract
Uniprot accession no.:
产品名称
Monoclonal Anti-POU5F1 (Oct4) antibody produced in mouse, ~1.0 mg/mL, clone PF-29, purified immunoglobulin, buffered aqueous solution
biological source
mouse
conjugate
unconjugated
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
PF-29, monoclonal
form
buffered aqueous solution
mol wt
antigen ~43 kDa
species reactivity
human
concentration
~1.0 mg/mL
technique(s)
immunocytochemistry: suitable
indirect ELISA: suitable
western blot: 0.5-1 μg/mL using NT2/D1 total cell extract
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Quality Level
Gene Information
human ... POU5F1(5460)
Application
Monoclonal Anti-POU5F1 (Oct4) antibody produced in mouse may be used in:
- immunoblotting
- immunocytochemistry
- enzyme-linked immunosorbent assay (ELISA)
Biochem/physiol Actions
Monoclonal Anti-POU5F1 (Oct4) recognizes human POU5F1/Oct4.
POU5F1 (POU class 5 homeobox 1) controls homeostasis of embryonic cell development and pluripotency. The POU5F1 gene is highly expressed in cancer cells and offers resistance against chemotherapy.
POU5F1 protein functions as an early developmental control gene. This protein has been recognized as fundamental in the maintenance of pluripotency of embryonic stem cells and primordial germ cells. POU5F1 protein has been proposed as a useful marker for germ cell tumors (GCTs) that can exhibit pluripotential, specifically seminoma/ dysgerminoma/germinoma and embryonal carcinoma (EC). POU5F1 has also been established as a marker for extragonadal germ cell tumors (GCT) and metastatic tumors.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
General description
Monoclonal Anti-POU5F1 (Oct4) (mouse IgG2a isotype) is derived from the hybridoma PF-29 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice, immunized with a peptide.
POU5F1 (POU class 5 homeobox 1) gene codes for a transcription factor that binds to octamer sequence. POU5F1 is mapped to human chromosome 6p21.33.
Immunogen
amino acids 20-37 of human POU5F1 peptide.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Preparation Note
For continuous use, store at 2-8 °C for up to one month. For extended storage, freeze at -20 °C in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.
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存储类别
10 - Combustible liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
法规信息
常规特殊物品
此项目有
Clinical significance of putative markers of cancer stem cells in gastric cancer: A retrospective cohort study.
Chen X L, et al.
Oncotarget, 7(38), 62049-62049 (2016)
Timothy D Jones et al.
The American journal of surgical pathology, 28(7), 935-940 (2004-06-30)
OCT4 (POU5F1) is a transcription factor expressed in embryonic stem and germ cells and is involved in the regulation and maintenance of pluripotency. It has been detected in primary testicular germ cell tumors with pluripotent potential, seminoma, and embryonal carcinoma.
Chemotherapeutics-induced Oct4 expression contributes to drug resistance and tumor recurrence in bladder cancer.
Lu C S, et al.
Oncotarget, 8(19), 30844-30844 (2017)
Ming-Tse Sung et al.
Human pathology, 37(6), 662-667 (2006-05-31)
Correctly diagnosing a metastatic germ cell tumor after chemotherapy may be challenging because of the diverse morphological manifestations of postchemotherapy tumors. Both OCT4 and CD30 are sensitive markers for the identification of primary embryonal carcinomas; however, loss of expression of
Minimum regions of genomic imbalance in stage I testicular embryonal carcinoma and association of 22q loss with relapse.
Gilbert D C, et al.
Genes Chromosomes Cancer, 50(3), 186-195 (2011)
商品
Quantitative and qualitative western blotting to validate knockdown by esiRNA.
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