P0084
Monoclonal Anti-Pinin antibody produced in mouse
~1.5 mg/mL, clone 5F1, purified immunoglobulin, buffered aqueous solution
别名:
Anti-DRS, Anti-PNN, Anti-SDK3, Anti-memA
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NACRES:
NA.41
UNSPSC Code:
12352203
Conjugate:
unconjugated
Clone:
5F1, monoclonal
Application:
ELISA (i), ICC, WB
Citations:
11
biological source
mouse
conjugate
unconjugated
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
5F1, monoclonal
form
buffered aqueous solution
mol wt
antigen ~140 kDa
species reactivity
monkey, bovine, human, canine
packaging
antibody small pack of 25 μL
concentration
~1.5 mg/mL
technique(s)
immunocytochemistry: suitable, indirect ELISA: suitable, western blot: 1-2 μg/mL using HeLa nuclear cell extract
isotype
IgG1
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... PNN(5411)
General description
Monoclonal Anti-Pinin (mouse IgG1 isotype) is derived from the hybridoma 5F1 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice. Pinin (PNN) is a serine/arginine-rich (SR)-related protein, that is found to localize to the cytoplasmic face of the desmosomal plaque in the convergence of intermediate filaments and to pin them to the desmosome. It consists of an Arg-Ser (RS) domain in its carboxyl terminus. This gene is mapped to human chromosome 14q.
Immunogen
synthetic peptide corresponding to amino acids 547-737 of human pinin, conjugated to KLH.
Application
Monoclonal Anti-Pinin antibody produced in mouse may be used in:
- enzyme-linked immunosorbent assay (ELISA)
- immunoblotting
- immunocytochemistry
Biochem/physiol Actions
Monoclonal Anti-Pinin recognizes human pinin. It crossreacts with monkey, bovine, and dog pinin.
Pinin (PNN) is mainly linked with the mature desmosomes of the epithelia. It plays a role in inducing junction formation and enhance cell aggregation. PNN transcription and protein levels were found to be reduced in renal and transitional cell carcinomas, suggesting its role as a tumor suppressor. PNN protein reduction by RNAi or knockdown resulted in loss of cell-cell adhesion as well as aberrant mouse development. It plays a key role in mRNA export, cell−cell adhesion, alternative splicing and cell migration. PNN also controls gene transcription.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Preparation Note
For extended storage, freeze at -20 °C in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if notused within 12 hours.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Loss of Pnn expression results in mouse early embryonic lethality and cellular apoptosis through SRSF1-mediated alternative expression of Bcl-xS and ICAD
Leu S, et al.
Journal of Cell Science, 125(13), 3164-3172 (2012)
Alvin Wong et al.
Plastic and reconstructive surgery, 130(5), 1012-1021 (2012-10-26)
Infantile hemangiomas can cause significant morbidity during proliferation, yet there is no U.S. Food and Drug Administration-approved treatment. They are believed to form from hemangioma stem cells, which differentiate toward a hemangioma endothelial cell phenotype. Recently, propranolol has demonstrated effectiveness
Reduction of Pnn by RNAi induces loss of cell-cell adhesion between human corneal epithelial cells
Joo JH, et al.
Molecular Vision, 11(1), 133-142 (2005)
Change in gene expression subsequent to induction of Pnn/DRS/memA: increase in p21 cip1/waf1
Shi Y, et al.
Oncogene, 20(30), 4007-4018 (2001)
Zhi Yu et al.
World journal of gastroenterology, 22(5), 1834-1843 (2016-02-09)
To investigate whether electroacupuncture (EA) at ST25 affects jejunal motility in vivo and if so, whether a sympathetic pathway is involved. Jejunal motility was assessed using a manometric balloon placed in the jejunum approximately about 3-5 cm away from the
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