P0117
Monoclonal Anti-PIAS3 小鼠抗
~2 mg/mL, clone PIA3, purified immunoglobulin, buffered aqueous solution
别名:
Anti-Protein Inhibitor of Activated STAT 3
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关于此项目
Conjugate:
unconjugated
Clone:
PIA3, monoclonal
Application:
immunocytochemistry
indirect ELISA
western blot
indirect ELISA
western blot
Species reactivity:
chicken, mouse, canine, human, bovine
Citations:
6
Technique(s):
immunocytochemistry: suitable
indirect ELISA: suitable
western blot: 2-4 μg/mL using HepG2 total cell extract
indirect ELISA: suitable
western blot: 2-4 μg/mL using HepG2 total cell extract
Uniprot accession no.:
产品名称
Monoclonal Anti-PIAS3 小鼠抗, ~2 mg/mL, clone PIA3, purified immunoglobulin, buffered aqueous solution
biological source
mouse
conjugate
unconjugated
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
PIA3, monoclonal
form
buffered aqueous solution
mol wt
antigen ~68 kDa (additional band at 44 kDa may be present)
species reactivity
chicken, mouse, canine, human, bovine
concentration
~2 mg/mL
technique(s)
immunocytochemistry: suitable
indirect ELISA: suitable
western blot: 2-4 μg/mL using HepG2 total cell extract
isotype
IgG1
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Quality Level
Gene Information
human ... PIAS3(10401)
mouse ... Pias3(229615)
Immunogen
synthetic peptide corresponding to amino acids 591-604 of human PIAS3.
Physical form
0.01M 磷酸缓冲盐溶液，pH 7.4，含 15mM 叠氮化钠。
Application
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunofluorescence (1 paper)
Immunohistochemistry (1 paper)
Immunofluorescence (1 paper)
Immunohistochemistry (1 paper)
Monoclonal Anti-PIAS3 antibody produced in mouse has been used in:
- enzyme linked immuno sorbent assay (ELISA)
- immunoblotting
- immunocytochemistry
Biochem/physiol Actions
Protein inhibitor of activated STAT protein 3 (PIAS3) and other members of this family can act as small ubiquitin-like modifier (SUMO) E3 ligases and interfere with the transcription activity of many transcription factors. PIAS3 binds to microphthalmia-associated transcription factor (MITF), a key DNA-binding protein. It blocks MITF DNA-binding activity and co-expression of MITF. PIAS3 in NIH 3T3 cells inhibits MITF-driven transcription activity.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
General description
PIAS3 encodes a member of the PIAS [protein inhibitor of activated STAT (signal transducer and activator of transcription)] family of transcriptional modulators. The protein functions as a SUMO (small ubiquitin-like modifier)-E3 ligase which catalyzes the
Protein inhibitor of activated STAT protein 3 (PIAS3) is an inhibitor of activated STAT3. The gene encodes for a 619-amino acid protein and has a molecular mass of 68 kDa. PIAS3 shares 55% identity with PIAS1 and PIASX proteins and 39% identity with PIASY. PIAS3 is widely expressed in various human tissues.
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存储类别
10 - Combustible liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
法规信息
常规特殊物品
此项目有
PIAS proteins and transcriptional regulation?more than just SUMO E3 ligases?
Sharrocks AD
Genes & Development, 20(7), 754-758 (2006)
PIAS3 may represent a potential biomarker for diagnosis and therapeutic of human colorectal cancer
Li H, et al.
Medical Hypotheses, 81(6), 1151-1154 (2013)
Zohar Yagil et al.
Journal of immunology (Baltimore, Md. : 1950), 182(4), 2168-2175 (2009-02-10)
Protein inhibitor of activated STAT3 (PIAS3) functions in vivo as a key molecule in suppressing the transcriptional activity of both microphthalmia transcription factor (MITF) and STAT3, two transcription factors that play a major role in the development, phenotypic expression, and
Akishi Onishi et al.
Nature neuroscience, 13(9), 1059-1065 (2010-08-24)
Selective expression of retinal cone opsin genes is essential for color vision, but the mechanism mediating this process is poorly understood. Both vertebrate rod and medium wavelength-sensitive (M) cone photoreceptors differentiate by repression of a short wavelength-sensitive (S) cone differentiation
A new role for the STAT3 inhibitor, PIAS3 a repressor of microphthalmia transcription factor
Levy C, et al.
The Journal of Biological Chemistry, 277(3), 1962-1966 (2002)
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