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P0157

酸性磷酸酶来源于马铃薯

Name: 酸性磷酸酶 来源于马铃薯
Brand: SIGMA

ammonium sulfate suspension, ≥200 units/mg protein (biuret)

别名:

(Orthophosphoric-monoester phosphohydrolase acid optimum), 酸性磷酸酶来源于马铃薯

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关于此项目

化学文摘社编号:

9001-77-8

EC Number:

232-630-9

UNSPSC Code:

12352204

EC 号:

3.1.3.2 (BRENDA, IUBMB)

MDL number:

MFCD00131847

eCl@ss:

42010102

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属性

form

ammonium sulfate suspension

specific activity

≥200 units/mg protein (biuret)

mol wt

111 kDa

foreign activity

Apyrase (ATPase) ≤5% of base activity

storage temp.

2-8°C

InChI

1S/C6H10O2/c1-3-4-8-5-6(2)7/h1,6-7H,4-5H2,2H3

InChI key

GZCWLCBFPRFLKL-UHFFFAOYSA-N

说明

General description

Acid phosphatase from potatoes is a 111 kDa diner consisting of two subunits at 41 and 35 kDa. This phosphatase has also been shown to cleave DNA.

Application

Phosphatase acid from potato has been used in a study to develop a method of efficient enzymatic hydrolysis of polyprenyl pyrophosphates. It has also been used in a study to investigate the kinetics of the hydrolysis of sodium p-nitrophenylphosphate and other phosphoric acid monoesters.

Biochem/physiol Actions

Acid phosphatases (APase) are a family of enzymes that non-specifically catalyze the hydrolysis of monoesters and anhydrides of phosphoric acid to produce inorganic phosphate at an optimum pH of 4 to 7.

Physical form

Suspension in 1.8 M (NH₄)₂SO₄, 10 mM MgCl₂, pH 5.5

Other Notes

One unit will hydrolyze 1.0 μmole of p-nitrophenyl phosphate per min at pH 4.8 at 37 °C.

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pictograms

GHS08

signalword

Danger

hcodes

H334

pcodes

P261 - P284 - P501

Hazard Classifications

Resp. Sens. 1

存储类别

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c