P0493
单克隆抗 PRMT5 小鼠抗
~2 mg/mL, clone PRMT5-21, purified immunoglobulin, buffered aqueous solution
别名:
Anti-Protein-Arginine Methyl Transferase 5
产品名称
单克隆抗 PRMT5 小鼠抗, ~2 mg/mL, clone PRMT5-21, purified immunoglobulin, buffered aqueous solution
biological source
mouse
conjugate
unconjugated
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
PRMT5-21, monoclonal
form
buffered aqueous solution
mol wt
antigen ~70 kDa by SDS-PAGE
species reactivity
mouse, hamster, bovine, canine, monkey, rat, human, chicken
concentration
~2 mg/mL
technique(s)
indirect ELISA: suitable
microarray: suitable
western blot: 2 μg/mL using whole cell extract of human melanoma cell line G361
isotype
IgG2a
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Quality Level
Gene Information
human ... PRMT5(10419)
Physical form
0.01M 磷酸缓冲盐溶液，pH 7.4，含 15mM 叠氮化钠。
Application
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)
Western Blotting (1 paper)
Monoclonal Anti-PRMT5 antibody produced in mouse has been used in enzyme-linked immunosorbent assay (ELISA) and immunoblotting.
Biochem/physiol Actions
Protein arginine methyltransferase 5 (PRMT5) participates in the symmetrical dimethylation of arginine residues. Homooligomerization of PRMT5 has an important role in its ability to methylate the myelin basic protein (MBP) protein. PRMT5 also binds the Janus kinases and are involved in an interferon-signaling pathway. It also helps to produce symmetrical ω-NG-dimethylarginine.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
General description
Monoclonal Anti-PRMT5 (mouse IgG2a isotype) is derived from the PRMT5-21 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from mice immunized with GST-PRMT5 containing the C-terminal region of the human PRMT5. Protein arginine methyltransferases (PRMT) 5 belongs to the type II arginine methyltransferases. PRMT5 (also known as Skb1Hs/janus kinase-binding protein 1 (JBP1) exists as homo-oligomeric complexes, which includes a dimer and tetramer. PRMT5 is found mainly in the cytoplasm. This gene is located on human chromosome 14q11.2.
Immunogen
GST-PRMT5 containing the C-terminal region of the human PRMT5 (amino acids 315-637).
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存储类别
12 - Non Combustible Liquids
wgk
nwg
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
常规特殊物品
此项目有
Ming Liu et al.
Theranostics, 10(10), 4437-4452 (2020-04-16)
The proto-oncogene c-Myc regulates multiple biological processes mainly through selectively activating gene expression. However, the mechanisms underlying c-Myc-mediated gene repression in the context of cancer remain less clear. This study aimed to clarify the role of PRMT5 in the transcriptional
Bing Yao et al.
Molecular cancer, 21(1), 119-119 (2022-05-28)
Prostate cancer (PCa) is the most frequently diagnosed malignancy in men, and its mechanism remains poorly understood. Therefore, it is urgent to discover potential novel diagnostic biomarkers and therapeutic targets that can potentially facilitate the development of efficient anticancer strategies.
LKB1 regulates PRMT5 activity in breast cancer
Lattouf H, et al.
International Journal of Cancer. Journal International Du Cancer, 144(3), 595-606 (2019)
Expression of protein arginine methyltransferase-5 in oral squamous cell carcinoma and its significance in epithelial-to-mesenchymal transition
Amano Y, et al.
Pathology international, 68(6), 359-366 (2018)
Liu Yang et al.
Theranostics, 11(8), 3742-3759 (2021-03-06)
Background: Protein arginine methyltransferase 5 (PRMT5) is a type II arginine methyltransferase that symmetrically di-methylates arginine residues on both histone and non-histone protein substrates. Accumulating evidence suggests that PRMT5 exerts its oncogenic properties in a wide spectrum of human malignancies.
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