产品名称
Protein A-Agarose from Staphylococcus aureus, lyophilized powder
form
lyophilized powder
matrix
Immobilized on 4% cross-linked beaded agarose
matrix activation
p-nitrophenyl chloroformate
matrix attachment
amino
matrix spacer
1 atom
capacity
30-40 mg/mL binding capacity (human IgG)
storage temp.
−20°C
Quality Level
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Application
Protein A-agarose is used for affinity chromatography, antibody purification and characterization, and protein A, G and L resins. Protein A-agarose has been used to study the effects of protein A immunoadsorption in patients with chronic dilated cardiomyopathy as well as to study multiple sclerosis and gastric cancer.
Physical form
Supplied as lyophilized powder stabilized with lactose.
Preparation Note
Swelling: 1 g swells to approx. 4 ml.
存储类别
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
法规信息
常规特殊物品
此项目有
Laurent Garderet et al.
Leukemia & lymphoma, 47(7), 1340-1347 (2006-08-23)
This study evaluated the feasibility of using dendritic cells (DCs) to generate, ex vivo, anti-tumor cytotoxic T lymphocytes (CTL) in patients with stage III multiple myeloma (MM). Nucleated cells from eight patients who had received chemotherapy (three of whom had
Xiaohong Shi et al.
Methods in molecular biology (Clifton, N.J.), 379, 137-148 (2007-05-16)
The membrane glycoproteins (Gn and Gc) of viruses in the family Bunyaviridae form projections on the virion envelope and are involved in virus entry and eliciting protective immunity. The glycoproteins are modified by N-linked glycosylation and accumulate in the Golgi
Guoxin Shen et al.
The Plant journal : for cell and molecular biology, 49(2), 228-237 (2007-01-24)
Animal CHIP proteins are chaperone-dependent E3 ubiquitin ligases that physically interact with Hsp70, Hsp90 and proteasome, promoting degradation of a selective group of non-native or damaged proteins in animal cells. The plant CHIP-like protein, AtCHIP, also plays important roles in
Andreas O Doesch et al.
Journal of clinical apheresis, 25(6), 315-322 (2010-09-09)
The objective of this study was to investigate functional effects of immunoadsorption (IA) in patients with chronic nonfamilial dilated cardiomyopathy (DCM) regarding clinical and humoral markers of heart failure. IA has been shown to induce early hemodynamic improvement in patients
Yu Ya Kit et al.
Biochemistry. Biokhimiia, 73(8), 950-956 (2008-09-09)
Immunoglobulins IgG and sIgA actively hydrolyzing histone H1 have been detected on analyzing proteolytic activity of antibodies isolated by chromatography on Protein A-agarose from blood serum of patients with multiple sclerosis and from colostrum of healthy mothers. These antibodies hydrolyze
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