P1034
异硫氰酸苯酯
Sigma Grade, 8.36 M, suitable for solid phase protein sequencing analysis, ≥99% (GC), liquid
别名:
PITC
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关于此项目
线性分子式:
C6H5NCS
化学文摘社编号:
分子量:
135.19
UNSPSC Code:
12352118
NACRES:
NA.31
PubChem Substance ID:
EC Number:
203-138-1
Beilstein/REAXYS Number:
471392
MDL number:
产品名称
异硫氰酸苯酯, Sigma Grade, 8.36 M, suitable for solid phase protein sequencing analysis, ≥99% (GC), liquid
InChI key
QKFJKGMPGYROCL-UHFFFAOYSA-N
InChI
1S/C7H5NS/c9-6-8-7-4-2-1-3-5-7/h1-5H
SMILES string
S=C=Nc1ccccc1
grade
Sigma Grade
assay
≥99% (GC)
form
liquid
concentration
8.36 M
refractive index
n20/D 1.6515 (lit.)
bp
218 °C (lit.)
mp
−21 °C (lit.)
density
1.132 g/mL at 20 °C (lit.)
suitability
suitable for solid phase protein sequencing analysis
storage temp.
2-8°C
Quality Level
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Application
异硫氰酸苯酯可用于通过自动埃德曼反应和之后的高效液相色谱分析（HPLC）技术，在亚皮摩尔和亚纳摩尔水平进行多肽和蛋白质测序。
用于伯胺和仲胺的衍生化试剂。用于通过 Edman 降解法进行多肽测序以及通过 HPLC 进行氨基酸分析。
General description
异硫氰酸苯酯属于带异硫氰酸基团的芳香族化合物类。它通常用作柱前衍生剂，用于形成苯硫代氨基甲酰基氨基酸，以便通过色谱技术进行测定。
Packaging
氩气下用火焰封装的 1mL 棕色安瓿瓶包装。
氩气下装瓶的 10mL 螺纹盖棕色样品瓶包装。
氩气下装瓶的 10mL 螺纹盖棕色样品瓶包装。
signalword
Danger
hcodes
Hazard Classifications
Acute Tox. 3 Oral - Resp. Sens. 1 - Skin Corr. 1B - Skin Sens. 1
存储类别
6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials
wgk
WGK 3
flash_point_f
190.4 °F - closed cup
flash_point_c
88 °C - closed cup
ppe
Faceshields, Gloves, Goggles, type ABEK (EN14387) respirator filter
法规信息
危险化学品
此项目有
R L Heinrikson et al.
Analytical biochemistry, 136(1), 65-74 (1984-01-01)
Methods for the quantitative derivatization of amino acids with phenylisothiocyanate and for the separation and quantitation of the resulting phenylthiocarbamyl derivatives by reverse-phase high-performance liquid chromatography are described. Phenylthiocarbamylation of amino acids proceeds smoothly in 5 to 10 min at
Examination of automated polypeptide sequencing using standard phenyl isothiocyanate reagent and subpicomole high-performance liquid chromatographic analysis
Tempst P and Riviere L
Analytical Biochemistry, 183(2), 290-300 (1989)
The Protein Protocols Handbook, 235(1), 48-62 (1996)
Additives for High Performance Applications: Chemistry and Applications
Additives for High Performance Applications: Chemistry and Applications, 235(1), 48-62 (2016)
H S Lu et al.
Archives of biochemistry and biophysics, 235(1), 48-62 (1984-11-15)
Automated solid-phase sequencing using 4-N,N-dimethylaminoazobenzene 4'-isothiocyanate (DABITC) double coupling or regular phenylisothiocyanate (PITC) degradation procedures have been investigated. Employing sensitive high-performance liquid chromatography for the identification of amino acid thiohydantoin derivatives (PTH and DABTH), both methods were capable of sequencing
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