P1063
邻苯二胺 二盐酸盐
tablet, 60 mg substrate per tablet
别名:
1,2-Phenylenediamine tablet, OPD, OPD Tablet
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关于此项目
线性分子式:
C6H4(NH2)2·2HCl
化学文摘社编号:
分子量:
181.06
UNSPSC Code:
12352204
NACRES:
NA.83
PubChem Substance ID:
EC Number:
210-418-7
Beilstein/REAXYS Number:
3912045
MDL number:
Quality Segment
form
tablet
mp
258 °C (dec.) (lit.)
storage temp.
2-8°C
SMILES string
Cl[H].Cl[H].Nc1ccccc1N
InChI
1S/C6H8N2.2ClH/c7-5-3-1-2-4-6(5)8;;/h1-4H,7-8H2;2*1H
InChI key
RIIWUGSYXOBDMC-UHFFFAOYSA-N
General description
邻苯二胺二盐酸盐（OPD）是一种显色底物，因为利用辣根过氧化物酶缀合物，因此可用于酶联免疫吸附试验（ELISA）程序。底物产生可溶的最终产物，其颜色为橙棕色，并且可以在450 m下通过分光光度计读取。可以用3N HCl或3M H2SO4终止OPD反应，并在492 nm处读取。
o-Phenylenediamine dihydrochloride (OPD) is a chromogenic substrate that can be used in enzyme-linked immunosorbent assay (ELISA) procedures as it utilizes horseradish peroxidase conjugates. The substrate produces a soluble end product that is orange-brown and can be read spectrophotometrically at 450 nm. The OPD reaction may be stopped with 3N HCl or 3M H2SO4 and read at 492 nm.
Application
Features and Benefits
Supplied as 50 or 100 tablets per box, individually wrapped with foil for easy storage, use, and safety.
Preparation Note
Dissolve one tablet in 0.05 M phosphate-citrate buffer, pH 5.0, to the desired concentration (typically an OPD concentration of 0.4 mg/ml is used). Add 40 μl of fresh 30% hydrogen peroxide per 100 ml of substrate buffer solution, immediately prior to use. Tablets are individually packaged in foil packets.
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signalword
Warning
Hazard Classifications
Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Carc. 2 - Eye Irrit. 2 - Muta. 2 - Skin Sens. 1
存储类别
11 - Combustible Solids
flash_point_f
Not applicable
flash_point_c
Not applicable
wgk
WGK 3