产品名称
垂体提取物 牛, lyophilized powder, suitable for cell culture
生物来源
bovine
质量水平
表单
lyophilized powder
效能
15 - 200 μg/mL EC50
质量
endotoxin tested
包装
pkg of 5 mg
技术
cell culture | mammalian: suitable
杂质
≤50 EU/mg
颜色
brown
溶解性
0.85% sodium chloride: soluble 1 vial/mL, clear to hazy, faintly yellow to dark yellow-orange
储存温度
2-8°C
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一般描述
Pituitary Extract, bovine (PEX) is an extract of bovine pituitary tissue containing growth factors.
应用
Pituitary Extract bovine has been used as a component in the Roswell Park memorial institute 1640 (RPMI-1640) to culture Rb-/- epithelial cells. It has also been used as a component in the Dulbecco′s modified Eagle′s medium (DMEM) for culturing Schwann cells.
生化/生理作用
Pituitary Extract, bovine (PEX) or BPE is essential for mitotic activity and promotes the growth of several types of mammalian cells. It favors the proliferation of keratocytes culture and bovine knee chondrocytes. BPE helps in the synthesis of collagen and glycosaminoglycans (GAGs) in bovine knee chondrocytes.
外形
Lyophilized from sodium phosphate buffer
法规信息
监管及禁止进口产品
此项目有
Yung-Chih Kuo et al.
Biotechnology progress, 21(6), 1708-1715 (2005-12-03)
In vitro cultivation of primary bovine knee chondrocytes (BKCs), using bovine pituitary extract (BPE) and porous scaffolds composed of polyglycolide (PGA) and 85/15 poly(lactide-co-glycolide) (PLGA), was investigated. Here, BPE was prepared from fresh bovine pituitaries, and cylindrical PGA/PLGA scaffolds with
Simon W Hayward et al.
Methods in molecular biology (Clifton, N.J.), 218, 17-33 (2003-03-06)
The ability to rescue viable prostate precursor tissue from Rb-/-fetal mice has allowed for the generation of Rb-/-prostate tissue and Rb-/-prostate epithelial cell lines. Herein, we provide a protocol for the rescue of urogenital precursor tissue from mouse embryos harboring
Zhong-Zhong Xu et al.
International journal of ophthalmology, 6(6), 758-765 (2014-01-07)
To investigate the effects of bovine pituitary extract on the proliferation of keratocytes and maintaining the keratocyte phenotype in vitro. Single keratocytes were isolated by enzyme digestion for in vitro culture. Three groups were designed according to the different culture
T M Brushart et al.
Experimental neurology, 247, 272-281 (2013-05-28)
Myelinating Schwann cells express distinct sensory and motor phenotypes as defined by their differing patterns of growth factor production (Hoke et al., 2006). The heterogeneous growth factor requirements of sensory and motor neurons, however, suggest that Schwann cell phenotype might
S L Hammond et al.
Proceedings of the National Academy of Sciences of the United States of America, 81(17), 5435-5439 (1984-09-01)
A serum-free medium with bovine pituitary extract as the only undefined supplement has been developed for long-term culture of human mammary epithelial cells. This medium supports serial subculture of normal cells for 10-20 passages (1:10 splits) without conditioning or special
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