产品名称
Polymyxin B ready made solution, Cell culture tested, 17 mg/mL in H2O
Quality Segment
form
liquid
concentration
17 mg/mL in H2O
storage temp.
2-8°C
General description
Polymyxin B (PMB) is a cyclic cationic lipopeptide antibiotic originally produced by soil bacterium Bacillus polymyxa. Polymyxin B binds specifically to gram negative bacteria by interacting with the anionic lipopolysaccharide (LPS, or endotoxin). Polymyxin B interferes with the outer membrane normal function causing leakage and enhanced uptake of the antibiotic.
Polymyxin B has several applications:
Working concentration:
The suggested concentration of PMB in cell culture mediums is 100U/mL. Therefore, it is recommended to dilute PMB ready made solution 1:1000.
Polymyxin B has several applications:
- It is used to neutralize LPS contamination in vitro and in vivo. Its neutralizing effect is dose-dependent. In vitro experiments showed that PMB inhibits a septic shock by endotoxin inactivation.
- Cell cultures can be treated with PMB (100U/mL) to avoid early contaminations. PMB is considered a broad spectrum antibiotic and is used to treat infections caused by bacteria resistant to a combination of Penicillin and Streptomycin.
- PMB is also used as negative and positive control in minimum microbicidal concentration (MIC) assay.
Working concentration:
The suggested concentration of PMB in cell culture mediums is 100U/mL. Therefore, it is recommended to dilute PMB ready made solution 1:1000.
存储类别
12 - Non Combustible Liquids
wgk
WGK 2