P1261
磷酸化酶 a 来源于兔肌肉
lyophilized powder, 20-30 units/mg protein
别名:
1,4-α-D-葡聚糖：正磷酸盐 α-D葡萄糖基转移酶
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关于此项目
化学文摘社编号:
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-878-8
MDL number:
Specific activity:
20-30 units/mg protein
Biological source:
rabbit muscle
biological source
rabbit muscle
Quality Level
form
lyophilized powder
specific activity
20-30 units/mg protein
purified by
2× crystallization
composition
Protein, 30-60% E1%/280
foreign activity
AMP-phosphatase, ATP-phosphatase, debrancher enzyme and phosphorylase phosphatase ≤0.1%, phosphoglucomutase ≤0.25%, phosphorylase b ≤30%
storage temp.
−20°C
General description
磷酸化酶 A 是糖原磷酸化酶的活性形式，其将糖原和正磷酸盐（Pi）转化为葡萄糖1-磷酸（G-1-P）。
Application
来自兔肌肉的磷酸化酶已用于混合糖原以制备极限糊精。它还被用于探测寡聚糖微阵列磷酸化酶活性分析中的阵列。
来自兔肌肉的磷酸化酶已被用于研究中评估齐墩果酸的分子机制。它也被用于研究中描述在 32P-ATP 存在下磷酸化酶 B 通过转化酶催化转化为磷酸化酶 A 的过程。
Biochem/physiol Actions
磷酸化酶参与糖原分解。磷酸化酶缺乏导致多种糖原贮积病和肝肿大。
磷酸化酶 A 可以被以下化合物抑制：多氯联苯、多氯联苯和多溴联苯。
通过磷酸化酶激酶的作用，二聚磷酸化酶 b 被转化为更有活性的四聚体磷酸化酶 a。
Physical form
冻干粉末，含有 β-甘油磷酸盐和 EDTA
Other Notes
在含有磷酸葡萄糖苷酶，NADP 和葡萄糖-6-磷酸脱氢酶的系统中测量，一个单位的酶在 pH 6.8，30℃ 下每分钟将糖原和正磷酸盐转化为 1.0 μmole α-D-1 葡萄糖 1-磷酸酯。（一个 μmolar 单位相当于约 45 Cori 单位。）
存储类别
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
法规信息
低风险生物材料
此项目有
Activity of porcine muscle glycogen debranching enzyme in relation to pH and temperature
Kyla-Puhju M, et al.
Meat Science, 69(1), 143-149 (2005)
Hypoglycemia in the toddler and child
Pediatric Endocrinology, Diabetes, and Metabolism, 920-955 (2014)
Ian J Tetlow et al.
The Plant cell, 16(3), 694-708 (2004-02-20)
Protein phosphorylation in amyloplasts and chloroplasts of Triticum aestivum (wheat) was investigated after the incubation of intact plastids with gamma-(32)P-ATP. Among the soluble phosphoproteins detected in plastids, three forms of starch branching enzyme (SBE) were phosphorylated in amyloplasts (SBEI, SBEIIa
Glycogen Phosphorylase Structures and Function
Fletterick, R. and S. Sprang
Accounts of Chemical Research, 15, 361-369 (1982)
R C Mead et al.
Biochimica et biophysica acta, 701(2), 173-179 (1982-02-18)
Polychlorinated biphenyls, polychlorinated biphenylols and polybrominated biphenyls inhibited both rabbit muscle phosphorylase a and phosphorylase b (1,4-alpha-D-glucan:orthophosphate alpha-d-glucosyltransferase, EC 2.4.1.1). The degree of inhibition was dependent upon the relative hydrophobicity of the compounds and steric hinderance. 2,4,5,2',4',5'-Hexabromobiphenyl and Firemaster BP-6
全球贸易项目编号
| 货号 | GTIN |
|---|---|
| P1261-10MG | 04061834354307 |
| P1261-25MG | 04061834354314 |
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