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Merck
CN

P1473

Sigma-Aldrich

PCR 100 bp 低梯形

for electrophoresis of PCR fragments

别名:

100bp maker, 100bp marker for agarose gel electrophoresis, 100pb ladder for agarose gel electrophoresis, DNA ladder, DNA marker, gel ladder, gel marker

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关于此项目

UNSPSC代码:
41141827
NACRES:
NA.25
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生物来源

synthetic

质量水平

等级

Molecular Biology

表单

liquid

用途

75 uses

适用性

suitable for electrophoresis (DNA)

储存温度

−20°C

一般描述

PCR 100 bp low ladder 专为测定聚合酶链反应(PCR)产生的DNA片段的大小而设计。
Sigma 的 PCR 100 bp 低标记包含来自 100-1,000 个碱基的 10 个 dsDNA 片段,均匀分布在 100 bp 的重复序列中。2ul 的梯状物应在凝胶上样缓冲液中稀释,然后上样到琼脂糖凝胶上的单一泳道中。

应用

PCR 100 bp Low Ladder已用于:
  • 在琼脂糖凝胶电泳中作为标准DNA标记,以获得约240 bp的DNA片段,用于聚合酶链反应(PCR)-变性梯度凝胶电泳(DGGE)分析
  • 从酶消化PCR混合物中可视化DNA
  • 估计PCR产物片段的分子量

其他说明

10 mM Tris-HCl (pH 8.0) 和 1.0 mM EDTA 溶液中提供 Sigma 的 PCR 100 bp 低标记物。
为获得最佳分离度,推荐琼脂糖凝胶浓度为 2.0%。
包含 10 个条带,100-1,000bp,精确的 100bp 间隔(梯状)重组重复序列。

储存分类代码

10 - Combustible liquids

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

法规信息

常规特殊物品
此项目有

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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Application of zinc oxide nanoparticles and plant growth promotingbacteria reduces genetic impairment under salt stress in tomato (Solanum lycopersicum L.`Linda?)
Hosseinpour A, et al.
A?r? : A?r? (Algoloji) Derne?i'nin Yay?n organ?d?r = The Journal of the Turkish Society of Algology, 10, 521-521 null
The relationships between performance traits and the bGH/Alu I and Pit-1/Hinf Ipolymorphisms in Holstein cows
Ozdemir M, et al.
Indian Journal of Animal Sciences (India) , 52, 186-191 null
Patricia Rivera et al.
Scientific reports, 10(1), 21154-21154 (2020-12-05)
Obesity has been firmly established as a major risk factor for common disease states including hypertension, type 2 diabetes mellitus, and chronic kidney disease. Increased body mass index (BMI) contributes to the activation of both the systemic and intra-tubular renin
Gbenga A Adewumi et al.
Frontiers in microbiology, 3, 436-436 (2013-01-15)
In this study, bacterial composition of iru produced by natural, uncontrolled fermentation of Parkia biglobosa seeds was assessed using culture-independent method in combination with culture-based genotypic typing techniques. PCR-denaturing gradient gel electrophoresis (DGGE) revealed similarity in DNA fragments with the
Susan Walker et al.
PloS one, 9(9), e106262-e106262 (2014-09-04)
In human adults, and animals, the Apelin-APJ ligand-receptor system is emerging as having a role in the pathogenesis of cardiovascular function and heart failure. The aim was to investigate expression, and regulation by oxygen, of the Apelin APJ receptor (APJ)

商品

Markers for gel electrophoresis aid size determination of DNA, PCR fragments, and RNA, staining well with common nucleic acid stains.

Choose the appropriate markers and ladders for nucleic acid size determination of samples separated by electrophoresis. Determine size of DNA, RNA and PCR-generated fragments using agarose or polyacrylamide gels.

选择适合的标记物和分子量标准,用于测定电泳分离样品的核酸大小。通过琼脂糖或聚丙烯酰胺凝胶检测DNA、RNA和PCR生成片段的大小。

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