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P1473

PCR 100 bp 低梯形

for electrophoresis of PCR fragments

别名:

100bp maker, 100bp marker for agarose gel electrophoresis, 100pb ladder for agarose gel electrophoresis, DNA ladder, DNA marker, gel ladder, gel marker

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关于此项目

NACRES:
NA.25
UNSPSC Code:
41141827
Biological source:
synthetic
Form:
liquid
Storage temp.:
−20°C
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biological source

synthetic

Quality Level

200

grade

Molecular Biology

form

liquid

usage

75 uses

suitability

suitable for electrophoresis (DNA)

storage temp.

−20°C

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General description

PCR 100 bp low ladder 专为测定聚合酶链反应（PCR）产生的DNA片段的大小而设计。
Sigma 的 PCR 100 bp 低标记包含来自 100-1,000 个碱基的 10 个 dsDNA 片段，均匀分布在 100 bp 的重复序列中。2ul 的梯状物应在凝胶上样缓冲液中稀释，然后上样到琼脂糖凝胶上的单一泳道中。

Application

PCR 100 bp Low Ladder已用于：
  • 在琼脂糖凝胶电泳中作为标准DNA标记，以获得约240 bp的DNA片段，用于聚合酶链反应（PCR）-变性梯度凝胶电泳（DGGE）分析
  • 从酶消化PCR混合物中可视化DNA
  • 估计PCR产物片段的分子量

Other Notes

10 mM Tris-HCl (pH 8.0) 和 1.0 mM EDTA 溶液中提供 Sigma 的 PCR 100 bp 低标记物。
为获得最佳分离度，推荐琼脂糖凝胶浓度为 2.0%。
包含 10 个条带，100-1,000bp，精确的 100bp 间隔（梯状）重组重复序列。

存储类别

10 - Combustible liquids

wgk

WGK 3

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

flash_point_f

No data available

flash_point_c

No data available

法规信息

常规特殊物品

此项目有

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
0000498712
0000498712
0000420136
0000420136
0000364605

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商品

Selecting DNA, RNA, and PCR Fragment Markers and Ladders for Gel Electrophoresis

Choose the appropriate markers and ladders for nucleic acid size determination of samples separated by electrophoresis. Determine size of DNA, RNA and PCR-generated fragments using agarose or polyacrylamide gels.

Gel Electrophoresis Markers

Markers for gel electrophoresis aid size determination of DNA, PCR fragments, and RNA, staining well with common nucleic acid stains.

选择用于凝胶电泳的DNA、RNA和PCR片段的标记物和分子量标准

选择适合的标记物和分子量标准，用于测定电泳分离样品的核酸大小。通过琼脂糖或聚丙烯酰胺凝胶检测DNA、RNA和PCR生成片段的大小。

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用于质粒制备、体外转录、mRNA 纯化和 LNP 制剂的工具，用于基于 mRNA 的疫苗和疗法开发。

Bioreagents for DNA/RNA Electrophoresis
Data Sheet - P1473

Instructions

全球贸易项目编号

货号GTIN
P1473-1VL04061835516230

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