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Merck
CN

P1585

PMA

for use in molecular biology applications, ≥99% (HPLC), Molecular Biology

别名:

佛波醇12-十四酸酯13-乙酸酯, NF-κB活化剂, PKC活化剂, 佛波醇酯, 巴豆油, 蛋白激酶C活化剂, 12-O-十四烷酰基佛波醇13-乙酸酯, 4β,9α,12β,13α,20-五羟基噻吩-1,6-二烯-3-酮12-十四烷酸酯13-乙酸酯, PMA, TPA

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关于此项目

经验公式（希尔记法）:
C36H56O8
化学文摘社编号:
16561-29-8
分子量:
616.83
UNSPSC Code:
12352200
NACRES:
NA.52
PubChem Substance ID:
24898219
MDL number:
MFCD00036736
Beilstein/REAXYS Number:
2407201

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产品名称

PMA, for use in molecular biology applications, ≥99% (HPLC), Molecular Biology

InChI

1S/C36H56O8/c1-7-8-9-10-11-12-13-14-15-16-17-18-29(39)43-32-24(3)35(42)27(30-33(5,6)36(30,32)44-25(4)38)20-26(22-37)21-34(41)28(35)19-23(2)31(34)40/h19-20,24,27-28,30,32,37,41-42H,7-18,21-22H2,1-6H3/t24-,27+,28-,30-,32-,34-,35-,36-/m1/s1

SMILES string

CCCCCCCCCCCCCC(=O)O[C@@H]1[C@@H](C)[C@@]2(O)C(C=C(CO)C[C@@]3(O)C2C=C(C)C3=O)C4C(C)(C)[C@]14OC(C)=O

InChI key

PHEDXBVPIONUQT-RGYGYFBISA-N

grade

Molecular Biology

assay

≥99% (HPLC)

form

(Lyophilisate)

mol wt

616.83  g/mol

storage condition

protect from light

solubility

DMSO: soluble (DMSO solutions can be stored dark at −20 °C for at least six months.)
ethanol: soluble (Dissolve in water-miscible organic solvent prior to preparing aqueous solutions)

storage temp.

−20°C

Quality Level

200

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Analysis Note

  • 刺激Jurkat细胞产生白介素2
  • 刺激T细胞
  • 蛋白激酶C (PKC)活化剂
  • NF-κB活化剂
  • 促细胞生长
  • 用于肿瘤启动子分析研究
  • 分子生物学级

Application

PMA已用于：
  • 胞内细胞分裂素检测
  • CellROX氧化应激分析
  • Huh7细胞刺激，以研究二肽基肽酶9 (DPP9)的重新分布
  • 用作慢性淋巴细胞性白血病(CLL)细胞B细胞受体(BCR)刺激的阳性对照
  • 单核细胞(THP -1)向巨噬细胞的分化

适用于
  • 作为标准品用于分子生物学研究
  • 肿瘤启动子分析研究
  • 促进细胞生长

Biochem/physiol Actions

有效的肿瘤启动子；体内和体外活化蛋白激酶C (PKC)。

Disclaimer

光敏感的，避光保存。

Features and Benefits

  • 刺激Jurkat细胞产生白介素2
  • 刺激T细胞
  • 蛋白激酶C (PKC)活化剂
  • NF-κB活化剂
  • 促细胞生长
  • 用于肿瘤启动子分析研究
  • 分子生物学级

General description

PMA（佛波醇12-十四酸酯13-乙酸酯）是一种蛋白激酶C（PKC）的激活剂和已知的肿瘤促进剂。PMA还表现出致裂（染色体断裂）活性。
佛波醇12-肉豆蔻酸酯13-乙酸酯可诱导PLD1（磷脂酶D1）活化，但不会诱导PLD2（磷脂酶D2）活化。

PMA通过蛋白激酶C (PKC)信号传导途径，充当以下机制相关的肿瘤启动子：

  • 基因转录
  • 细胞生长
  • 细胞分化
  • 细胞程序性死亡
  • 免疫途径
  • 受体脱敏

佛波醇12-肉豆蔻酸酯13-乙酸酯用于研究：

  • 生长因子
  • 激素
  • 细胞因子介导的细胞生长和分化调控

signalword

Danger

Hazard Classifications

Acute Tox. 1 Dermal - Acute Tox. 1 Inhalation - Acute Tox. 2 Oral - Carc. 2 - Eye Dam. 1 - Resp. Sens. 1 - Skin Corr. 1B - Skin Sens. 1

存储类别

6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials

wgk

WGK 3

ppe

dust mask type N95 (US), Eyeshields, Gloves

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
0000521596
0000521596
0000496763
0000496763
0000497070

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  1. How can I dissolve Phorbol 12-myristate 13-acetate, Product P1585?

    Phorbol 12-myristate 13-acetate (PMA) is soluble in DMSO at a 20 mM concentration.  It is also soluble in ethanol. 

  2. What is the stability of stock solutions of Phorbol 12-myristate 13-acetate, Product P1585, and how should I store them?

    Stock solutions in DMSO can be stored in the dark as aliquots at -20°C for at least six months.

  3. How much Phorbol 12-myristate 13-acetate, Product P1585, product should I use?

    A concentration of 50 ng/ml was used on Jurkat cells.

  4. There isn't any powder in the Phorbol 12-myristate 13-acetate, Product P1585, vial?

    The product is usually a clear film on the walls of the vial and may not be easily visible.

  5. What is the storage temperature for Phorbol 12-myristate 13-acetate, Product P1585?

    Phorbol 12-myristate 13-acetate should be stored at -20°C.

  6. What is the difference between Phorbol 12-myristate 13-acetate, Product P8139 and molecular biology grade Product P1585?

    Product P1585 has been tested and found suitable for stimulation of interleukin 2 production of Jurkat cells. Product P8139 has not tested in this application.

  7. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  8. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  9. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  10. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  11. How is Product P1585, Phorbol-12-myristate-13-acetate, packaged?

    P1585-1MG, P1585-10MG and P1585-25MG come to us pre-packaged by our supplier; all three sizes are packaged the same way. The packaging consists of an amber serum vial with a septum and an aluminum crimp cap. The septum has a PTFE (Teflon) liner, so the material inside the vial could only come in contact with glass and Teflon.

  12. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

The immune responses of central and effector memory BCG-specific CD4+ T cells in BCG-vaccinated PPD+ donors were modulated by Treg cells
Li L, et al.
Immunobiology, 216(4), 477-484 (2011)
Tumour promoter phorbol-12-myristate-13-acetate induces chromosomal damage via indirect action.
I Emerit et al.
Nature, 293(5828), 144-146 (1981-09-10)
Identification of a potential biomarker for FABP4 inhibition: the power of lipidomics in preclinical drug testing
Suhre K, et al.
Journal of Biomolecular Screening, 16(5), 467-475 (2011)
Biological properties and measurement of phospholipase D activation by ADP-ribosylation factor (ARF)
Cockcroft S, et al.
Methods in Enzymology, 329, 355-372 (2001)
Proteomic analysis of B-cell receptor signaling in chronic lymphocytic leukaemia reveals a possible role for kininogen
Kashuba E, et al.
Journal of Proteomics, 91(2), 478-485 (2013)
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相关内容

Phorbol 12-myristate 13-acetate Molecular Biology Reagent Protocol

T-cell activation is normally triggered by the interaction of a cell surface receptor to its specific ligand molecule

Data Sheet - P1585

PMA, through its activation of PKC, can activate T-cells and stimulate low-level production of IL-2. When Jurkat cells are stimulated by PMA and a co-stimulator, such as PHA, IL-2 production is strongly enhanced2.

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