Protein phosphatase 2C (PP2C) is a member of protein phosphatases, Mg²⁺/Mn²⁺ dependent (PPM) family. PP2C is a monomeric enzymes found in both prokaryotes and eukaryotes.

In Arabidopsis, protein phosphatase 2C (PP2C) negatively regulates plant hormone, abscisic acid (ABA) signal transduction. In eukaryotes, this protein modulates the cell cycle by reversing the activating phosphorylation of cyclin-dependent protein kinases (CDKs). PP2C acts as a crucial physiological regulators of cell growth and of cellular stress signaling.

Solution in 50 mM Tris-HCl, pH 7.0, containing 14 mM 2-mercaptoethanol, 1 mM benzamidine, 0.1 mM PMSF, 1 mM EDTA, and 50% glycerol

One unit will release 1.0 nanomole of phosphate from ³²P-labeled myelin basic protein at pH 7.0 at 30 °C