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安全信息

P1860

Sigma-Aldrich

Protease Inhibitor Cocktail

for use in tissue culture media, DMSO solution

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别名:
Protease Inhibitor Solution, protease inhibitor
EC 号:
200-664-3
MDL编号:
MFCD00677817
NACRES:
NA.77

质量水平

200

形式

DMSO solution

运输

dry ice

储存温度

−20°C

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相关类别

一般描述

蛋白酶抑制剂复合物混合多种抑制剂， 防止组织培养基中的分泌蛋白被蛋白酶降解。
Protease Inhibitor Cocktail demonstrated non-toxicity towards the following cell lines after 48 hours exposure:
A431, CHO, COS, HepG2, and HeLa adherent cell lines
Jurkat and HL-60 cell lines grown in suspension

特异性

抑制丝氨酸、半胱氨酸、和天冬氨酸蛋白酶和氨肽酶

应用

本品 专门针对组织培养基优化， 在培养基中作用48小时后可换新液，确保持续性 抑制蛋白酶。
Protease Inhibitor Cocktail has been used:
  • as a supplement in supernatants and lysates for ex vivo infection experiments using human precision-cut lung slices (PCLS)
  • to inhibit proteolysis by trypsin to prove that proteolysis is responsible for restoration of catalysis of corona-inhibited nanozymes
  • in trypsin solution for trypsin-inhibited studies
  • as a component in radioimmunoprecipitation assay (RIPA) buffer to lyse leukocytes for western blot

生化/生理作用

Protease inhibitor cocktail is designed for use in tissue culture media. It is recommended as an additive to tissue culture media to safeguard against the degradation of secreted proteins originating from the cultured tissue.

特点和优势

  • 广泛的特异性
  • 无毒性：作用48小时后，本品对贴壁细胞系A431、CHO、COS、HepG2和HeLa，以及悬浮细胞系Jurkat和HL-60无毒。
  • 不含金属螯合剂：确保兼容下游应用
  • 方便包装：1 mL玻璃瓶包装
  • 易于使用：在组织培养基中以1：200或更高的稀释度使用，以防止分泌蛋白被蛋白酶水解。

组分

抑肽酶
抑氨肽酶
E-64
亮肽素
胃蛋白酶抑制剂A

注意

孵育48小时后，产物对贴壁细胞系A431、CHO、COS、HepG2和HeLa，以及悬浮培养的Jurkat和HL-60细胞系无毒。

数量

在组织培养基中以1：200或更高的稀释度使用，以防止分泌蛋白的蛋白水解降解。

外形

溶于DMSO中（D 2650，Hybri-Max）。

其他说明

For R&D use only. Not for drug, household, or other uses. Please consult the Safety Data Sheet for information regarding hazards and safe handling practices.

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储存分类代码

10 - Combustible liquids

WGK

WGK 1

闪点(°F)

188.6 °F

闪点(°C)

87 °C

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