产品名称
Protease Inhibitor Cocktail, for use in tissue culture media, DMSO solution
form
DMSO solution
solubility
water: soluble
shipped in
dry ice
storage temp.
−20°C
Quality Level
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Application
本品 专门针对组织培养基优化, 在培养基中作用48小时后可换新液,确保持续性 抑制蛋白酶。
蛋白酶抑制剂混合物的应用示例:
- 作为补充剂掺入上清液和裂解物,用于人精密切割肺切片(PCLS)的离体感染实验
- 用于抑制胰蛋白酶介导的蛋白水解,证明蛋白水解可恢复被蛋白冠抑制的纳米酶的催化活性
- 加入胰蛋白酶溶液用于胰蛋白酶抑制研究
- 作为放射性免疫沉淀分析(RIPA)缓冲液组分,裂解白细胞用于蛋白质印迹分析
Biochem/physiol Actions
抑制丝氨酸、半胱氨酸、和天冬氨酸蛋白酶和氨肽酶
蛋白酶抑制剂混合物专用于组织培养基。适合作为组织培养基添加剂,保护培养的组织分泌蛋白免遭降解。
Disclaimer
孵育48小时后,产物对贴壁细胞系A431、CHO、COS、HepG2和HeLa,以及悬浮培养的Jurkat和HL-60细胞系无毒。
Features and Benefits
- 广泛的特异性
- 无毒性:作用48小时后,本品对贴壁细胞系A431、CHO、COS、HepG2和HeLa,以及悬浮细胞系Jurkat和HL-60无毒。
- 不含金属螯合剂:确保兼容下游应用
- 方便包装:1 mL玻璃瓶包装
- 易于使用:在组织培养基中以1:200或更高的稀释度使用,以防止分泌蛋白被蛋白酶水解。
General description
蛋白酶抑制剂复合物混合多种抑制剂, 防止组织培养基中的分泌蛋白被蛋白酶降解。
蛋白酶抑制剂混合物处理以下细胞系48小时后无毒性:
A431、CHO、COS、HepG2和HeLa贴壁细胞系
Jurkat和HL-60悬浮细胞系
A431、CHO、COS、HepG2和HeLa贴壁细胞系
Jurkat和HL-60悬浮细胞系
Other Notes
仅供研发使用。&不可用于药物、家庭或其他用途。有关危险和安全处理方法的信息,请参阅安全数据说明书。
抑肽酶
抑氨肽酶
E-64
亮肽素
胃蛋白酶抑制剂A
抑氨肽酶
E-64
亮肽素
胃蛋白酶抑制剂A
Physical form
溶于DMSO中(D 2650,Hybri-Max)。
Preparation Note
在组织培养基中以1:200或更高的稀释度使用,以防止分泌蛋白的蛋白水解降解。
存储类别
10 - Combustible liquids
wgk
WGK 1
flash_point_f
188.6 °F
flash_point_c
87 °C
法规信息
常规特殊物品
低风险生物材料
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相关内容
Select different protease inhibitor types based on your needs to prevent protein degradation during isolation and characterization and safeguard proteins in sample prep.
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