一般描述
Phenol:Chloroform:Iso-amyl alcohol (125:24:1) is used for the purification of total RNA from mixtures of crude cell extracts and can eliminate the need for a time consuming ultracentrifugation step. The addition of phenol, chloroform, and isoamyl can be employed to aid in the separation of lipids and cellular debris, leading to the transfer of these components to the organic phase. Meanwhile, the genomic DNA (gDNA) remains in the aqueous phase, effectively isolating it from the other substances.
应用
Phenol:Chloroform:Iso-amyl alcohol (125:24:1) has been used:
- for the extraction and purification of RNA.
- to isolate RNA from Arabidopsis siliques.
- to extract proteins and DNA from polysomal and non-polysomal fractions.
- in TRIzol methods to prepare and extract miRNAs from cells, tissue, and whole flies.
警示用语：
Danger
危险分类
Acute Tox. 3 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Aquatic Chronic 2 - Carc. 2 - Eye Dam. 1 - Muta. 2 - Repr. 2 - Skin Corr. 1B - STOT RE 1 Oral - STOT RE 2
靶器官
Liver,Kidney, Nervous system,Kidney,Liver,Skin
储存分类代码
6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials
WGK
WGK 3
闪点(°F)
175.0 °F - closed cup
闪点(°C)
79.44 °C - closed cup
个人防护装备
Faceshields, Gloves, Goggles, type ABEK (EN14387) respirator filter
法规信息
新产品
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Small untranslated RNA antitoxin in Bacillus subtilis.
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Journal of Bacteriology, 187(19), 6641-6650 (2005)
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