form
lyophilized powder
specific activity
≥60 units/mg protein
composition
Protein, 20-40% biuret
foreign activity
ATPase ≤0.5%, phosphorylase a ≤1%, phosphorylase b ≤5%
storage temp.
−20°C
General description
磷酸化酶激酶包含四个亚基,每个亚基含有α、β、γ以及sigma成分。该sigma成分结合4个钙分子,称为钙调蛋白,而其γ单位可用作催化亚基。
Application
来自兔肌肉的磷酸化酶激酶已应用于一项研究,以评估糖原磷酸化酶的特征。 此外,它还应用于研究肌动蛋白对不同形式肌磷酸化酶激酶的活化作用。
Physical form
含有(NH4)2SO4、蔗糖、β-甘油磷酸盐和二硫赤藓糖醇的冻干粉
Other Notes
在30°C、pH 7.7和ATP存在的条件下,一个单位每分钟可将磷酸化酶B转化为1.0 μ摩尔磷酸化酶。
存储类别
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
法规信息
低风险生物材料
此项目有
Monica Balsera et al.
Planta, 237(2), 619-635 (2012-12-12)
Uncovered in studies on photosynthesis 35 years ago, redox regulation has been extended to all types of living cells. We understand a great deal about the occurrence, function, and mechanism of action of this mode of regulation, but we know little
Laura A Lane et al.
Molecular & cellular proteomics : MCP, 11(12), 1768-1776 (2012-09-12)
Phosphorylase kinase (PhK), a 1.3 MDa enzyme complex that regulates glycogenolysis, is composed of four copies each of four distinct subunits (α, β, γ, and δ). The catalytic protein kinase subunit within this complex is γ, and its activity is
K F Chan et al.
The Journal of biological chemistry, 257(10), 5956-5961 (1982-05-25)
Aspects of the molecular interaction and subunit structure of rabbit skeletal muscle phosphorylase kinase, (alpha beta gamma delta)4, were investigated. Exogenous addition of the delta subunit (calmodulin) stimulated the activities of nonactivated phosphorylase kinase and the alpha gamma delta complex
全球贸易项目编号
| 货号 | GTIN |
|---|---|
| P2014-2.5KU | 04061832925103 |
| P2014-1KU | 04061832925097 |