Merck
CN
所有图片(1)

文件

安全信息

P2069

Sigma-Aldrich

苯酚:氯仿:异戊醇 25:24:1，10 mM Tris（pH 8.0）、1 mM EDTA饱和

Supplied with Equilibration buffer, for molecular biology

登录查看公司和协议定价
所有图片(1)
别名:
DNA extraction
NACRES:
NA.52

等级

for molecular biology

质量水平

200

检测方案

≥99.0%

形式

liquid

pH值（酸碱度）

6.5-6.9(pH of phenolic phase)
7.8-8.2(pH of phenolic phase after the addition of Equilibration Buffer)

应用

agriculture

储存温度

2-8°C

应用

对于需要更高 pH 值的应用，例如大的完整基因组 DNA的分离，建议添加平衡缓冲液。
苯酚：氯仿：异戊醇已用于制备 RNA，用于研究从心肌活检中分离总 RNA。它还被用于在人雄激素受体（HUMARA） 克隆性检测中提取 DNA。

包装

随配 P3803 和一瓶单独的平衡缓冲液，用于将苯酚相调节至 pH 8.0 ± 0.2。

适用性

这批苯酚：氯仿：异戊醇 25:24:1 采用从大肠杆菌中提取 的pBR322 DNA 的粗制品进行了测试。 根据琼脂糖凝胶电泳评估，提取的 DNA 没有降解或浓度降低。 采用相同的操作程序处理纯化的 pBR322 DNA 和 λ Hind III 消化 DNA。 根据琼脂糖凝胶电泳评估，纯化的制剂没有检测到降解或浓度降低。

制备说明

将小瓶平衡缓冲液的全部内容物添加到大瓶苯酚：氯仿：异戊醇中。 在使用前轻柔地混合并使相实现分离，大约 2-4 小时。 这会将苯酚溶液的 pH 值从 6.7± 0.2 调整到 8.0± 0.2。

其他说明

添加平衡缓冲液后，产品可稳定 6 个月，而不添加缓冲液可稳定 2 年。 在添加平衡缓冲液后，在 -20 °C 下避光储存可将保质期延长至 1 年以上。

相关产品

产品编号
说明
价格

警示用语：

Danger

危险分类

Acute Tox. 3 Oral - Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Aquatic Chronic 2 - Carc. 2 - Eye Dam. 1 - Muta. 2 - Repr. 2 - Skin Corr. 1B - STOT RE 1 Oral - STOT RE 2 - STOT SE 3

靶器官

Central nervous system

储存分类代码

6.1A - Combustible, acute toxic Cat. 1 and 2 / very toxic hazardous materials

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

法规信息

新产品

分析证书(COA)

输入产品批号来搜索 分析证书(COA) 。批号可以在产品标签上"批“ （Lot或Batch）字后找到。

已有该产品？

为方便起见，与您过往购买产品相关的文件已保存在文档库中。

访问文档库

难以找到您所需的产品或批次号码？

在网站页面上，产品编号会附带包装尺寸/数量一起显示（例如：T1503-25G）。请确保 在“产品编号”字段中仅输入产品编号 (示例: T1503).

示例

T1503
货号
-
25G
包装规格/数量

其它示例：

705578-5MG-PW

PL860-CGA/SHF-1EA

MMYOMAG-74K-13

1000309185

输入内容 1.000309185)

遇到问题？欢迎随时联系我们技术服务 寻求帮助

批号可以在产品标签上"批“ （Lot或Batch）字后面找到。

Aldrich 产品

  • 如果您查询到的批号为 TO09019TO 等，请输入去除前两位字母的批号：09019TO。

  • 如果您查询到的批号含有填充代码（例如05427ES-021），请输入去除填充代码-021的批号：05427ES。

  • 如果您查询到的批号含有填充代码（例如 STBB0728K9），请输入去除填充代码K9的批号：STBB0728。

未找到您寻找的产品？

部分情况下，可能未在线提供COA。如果搜索不到COA，可在线索取。

索取COA

  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. There are two layers in the bottle of Product P2069, Phenol:Chloroform:Isoamyl Alcohol 25:24:1; which layer should I use?

    The lower phase is the phase that is to be used.

  4. There is an Equilibration Buffer (Product B5658) that is included with Product P2069, Phenol:Chloroform:Isoamyl Alcohol 25:24:1.  Is B5658 also available separately?

    No, it is only available as part of P2069.

  5. I ordered Product P2069, Phenol:Chloroform:Isoamyl Alcohol 25:24:1; I got one bottle of the Phenol:Chloroform:Isoamyl Alcohol and one bottle of B5658, Equilibration buffer. When and how am I supposed to use the B5658?

    B5658 is a Tris buffer that is used to raise the pH of the phenol:chloroform:isoamyl alcohol mixture, to make it suitable for isolation of genomic DNA. The contents of the two bottles need to be well mixed. Then allow the mixture to separate; this may take 2-4 hours. This procedure should raise the pH of the phenol phase from 6.7 to about 8.0.  Only one "treatment" is needed. This solution should be stable for up to 12 months.

  6. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  7. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  8. I see that you list two equilibrated Phenol:Chloroform:Isoamyl alcohol products. When should I use Product P3803, and when should I use Product P2069?

    In short, P3803 is for RNA work, and P2069 is for DNA work. Product P2069 consists of one bottle of Product P3803 and one bottle of Product B5658. The B5658 is added to the P3803 to raise the pH of the organic phase from 6.7 to 8.0; pH 8.0 is not suitable for RNA work, because RNA can degrade at the higher pH.

  9. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

Yongzhong Wang et al.
Microcirculation (New York, N.Y. : 1994), 13(3), 187-197 (2006-04-22)
Angiogenic therapies in animals have demonstrated the development of new blood vessels within ischemic myocardium. However, results from clinical protein and gene angiogenic trials have been less impressive. The present study aimed to investigate the expression of angiogenic genes in
Amber L Scott et al.
Molecular biology and evolution, 34(10), 2690-2703 (2017-09-29)
Polyploidization events have occurred during the evolution of many fungi, plant, and animal species and are thought to contribute to speciation and tumorigenesis, however little is known about how ploidy level contributes to adaptation at the molecular level. Here we
Martin Häring et al.
Frontiers in behavioral neuroscience, 9, 235-235 (2015-09-22)
The endocannabinoid (eCB) system possesses neuromodulatory functions by influencing the release of various neurotransmitters, including γ-aminobutyric acid (GABA) and glutamate. A functional interaction between eCBs and the serotonergic system has already been suggested. Previously, we showed that cannabinoid type-1 (CB1)
Laura Cook et al.
Cellular and molecular gastroenterology and hepatology, 9(3), 485-506 (2019-12-04)
Bacterial flagellin is an important antigen in inflammatory bowel disease, but the role of flagellin-specific CD4+ T cells in disease pathogenesis remains unclear. Also unknown is how changes in intestinal microbiome intersect with those in microbiota-specific CD4+ T cells. We
Jacob F Warner et al.
Methods in molecular biology (Clifton, N.J.), 2219, 231-240 (2020-10-20)
The sea anemone Nematostella vectensis is an emerging research model to study embryonic development and regeneration at the molecular and global transcriptomic level. Transcriptomics analysis is now routinely used to detect differential expression at the genome level. Here we present
查看所有相关文献

实验方案

Reverse Transcription Protocol

Method for reverse transcription of RNA into DNA. Uses a premixed reagent that contains reverse transcriptase, dNTPs, primers, RNase inhibitor and buffer. Fast generation of cDNA.

逆转录实验方案

将RNA逆转录成DNA的方法。使用含有逆转录酶、dNTP、引物、RNase抑制剂和缓冲液的预混试剂。快速合成cDNA。

我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.

联系技术服务部门