P2545
蛋白 A-琼脂糖
saline suspension
别名:
Protein A resin
产品名称
蛋白 A-琼脂糖, saline suspension
biological source
Staphylococcus aureus
form
saline suspension
extent of labeling
~2 mg per mL
matrix activation
cyanogen bromide
matrix attachment
amino
matrix spacer
1 atom
capacity
≥15 mg/mL binding capacity (human IgG)
storage temp.
2-8°C
Quality Level
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Application
Protein A-agarose 用于亲和层析、抗体纯化和表征以及 Protein A、G 和 L 树脂。蛋Protein A-agarose 已被用于研究慢性扩张型心肌病患者的蛋白 A 免疫吸附作用以及研究多发性硬化和胃癌。
蛋白 A-琼脂糖已用于小鼠成纤维细胞 L-cell 和人胚肾 (HEK) 293 细胞中 Wnt3a 和音猬因子 (Shh) 的脯氨酰羟化酶结构域 2 N27 细胞的 S63 和 NIH3T3 成纤维细胞的蛋白磷酸酶和张力蛋白同源物 (PTEN) 蛋白的
- 免疫沉淀
Biochem/physiol Actions
蛋白 A 与 Fcγ（恒定区）以及 IgG 的 Fab（抗原结合片段）结合。蛋白 A 琼脂糖树脂在商业上用于基于亲和力的纯化。
General description
蛋白 A 是来自 金黄色葡萄球菌 的 42-kDa 蛋白，具有 5 个免疫球蛋白结合域。
Physical form
混悬于含防腐剂的0.5 M NaCl中
存储类别
10 - Combustible liquids
wgk
WGK 3
ppe
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
法规信息
常规特殊物品
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D J McCool et al.
The Biochemical journal, 341 ( Pt 3), 593-600 (1999-07-27)
Molecular chaperones are presumed to associate with large secretory mucin glycoproteins during their synthesis in the endoplasmic reticulum (ER), but have not been identified to date. We decided to look for possible involvement of the chaperones calreticulin (CRT) and calnexin
Madhu Chhanda Mohanty et al.
Developmental and comparative immunology, 26(4), 385-391 (2002-03-13)
Meriones unguiculatus commonly known as gerbils are widely used as animal models for a variety of parasitic infections such as Brugia malayi, Entamoeba histolytica, Giardia duodenalis, Toxoplasma gondi, Helicobacter pylori, Strongyloides stercoralis and Echinococcus multilocularis. Groups of BALB/c mice, gerbils
Hsp90 Co-chaperone p23 contributes to dopaminergic mitochondrial stress via stabilization of PHD2: Implications for Parkinson?s disease
Rane A, et al.
Neurotoxicology, 65, 166-173 (2018)
Evaluation of a novel methacrylate-based protein a resin for the purification of immunoglobulins and Fc-fusion proteins
McCaw TR, et al.
Biotechnology Progress, 30(5), 1125-1136 (2014)
Membrane targeting of palmitoylated Wnt and Hedgehog revealed by chemical probes
Gao X, et al.
Febs Letters, 585(15), 2501-2506 (2011)
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