一般描述
特异性
应用
- in radioimmunoprecipitation assay buffer to lyse the avian crystalline lens samples for western blot analysis
- in phosphate buffer solution (PBS) for grinding recombinant protein expressed leaves to purify virus-like particles (VLPs)
- in PBS to dissolve lyophilized skin secretion sample of A. loloensis for peptide purification
- in 0.1 M NaCl solution to wash and collect skin secretions of frog
生化/生理作用
组分
AEBSF，2 mM
抑肽酶(Aprotinin)，0.3 μM
抑氨肽素(Bestatin)，116 μ
E-64，14 μM
EDTA，1 mM
亮抑酶肽(Leupeptin)，1 μM
每种组分各具特定的抑制特性。
注意
数量
并非所有提取物都含有相同水平的内源酶，并且可能需要调整所需的混合液用量。
制备说明
其他说明
相关产品
警示用语：
Warning
危险声明
危险分类
Eye Irrit. 2 - Skin Irrit. 2
储存分类代码
11 - Combustible Solids
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
分析证书(COA)
输入产品批号来搜索 分析证书(COA) 。批号可以在产品标签上"批“ （Lot或Batch）字后找到。
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如何查找产品货号
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示例
其它示例：
705578-5MG-PW
PL860-CGA/SHF-1EA
MMYOMAG-74K-13
1000309185
输入内容 1.000309185)
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如何查找COA批号
批号可以在产品标签上"批“ （Lot或Batch）字后面找到。
Aldrich 产品
如果您查询到的批号为 TO09019TO 等，请输入去除前两位字母的批号：09019TO。
如果您查询到的批号含有填充代码（例如05427ES-021），请输入去除填充代码-021的批号：05427ES。
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部分情况下，可能未在线提供COA。如果搜索不到COA，可在线索取。
How much of Product P2714, Protease Inhibitor Cocktail, needs to be used in cell lysates?
One bottle is recommended for the inhibition of proteases present in a maximum of 20 grams of cell extract.
How should I dissolve and store Product P2714, Protease Inhibitor Cocktail?
You can dissolve the product in 10 ml of water to make a 10X stock solution and then dilute 1:10 for use. Aliquots can be stored at -20 C for 1 month.
How much of Product P2714, Protease Inhibitor Cocktail, should I use with serum?
Dilute the contents of the vial in 10 mL of water, yielding a 10X initial concentration . Then dilute the stock 1:10 in the serum (1 part of inhibitor solution to 10 parts of serum). This would be a 1:100 dilution (containing the concentrations of each inhibitor as listed on the data sheet) in the serum. This is true for most cell lysates, serum, and culture media.
Can Product P2714, Protease Inhibitor Cocktail, be used with ELISA?
Yes, because it is water based.
Can Product P2714, Protease Inhibitor Cocktail,be used with Nickel columns?
No because it contains EDTA.
Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.
How do I get lot-specific information or a Certificate of Analysis?
The lot specific COA document can be found by entering the lot number above under the "Documents" section.
How do I find price and availability?
There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote. USA customers: 1-800-325-3010 or view local office numbers.
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
What is the difference between Protease Inhibitors S8820 and P2714?
S8820 is the tablet version of P2714.
My question is not addressed here, how can I contact Technical Service for assistance?
Ask a Scientist here.
商品
While aprotinin and bovine pancreatic trypsin inhibitor (BPTI) are the same protein sequence, the term aprotinin is typically used when describing the protein derived from bovine lung.
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