产品名称
蛋白酶抑制剂混合物粉末, for general use, lyophilized powder
biological source
bovine lung
Quality Level
form
lyophilized powder
solubility
water: soluble
storage temp.
−20°C
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General description
该蛋白酶抑制剂混合物已针对一般用途进行了优化和测试。内含水溶性蛋白酶抑制剂,可有效抑制丝氨酸、半胱氨酸和金属蛋白酶。该产品以冻干粉形式提供。
Application
蛋白酶抑制剂混合物粉末可作为组分:
- 加入放射性免疫沉淀分析缓冲液,以裂解禽类晶状体样本用于蛋白质免疫沉淀分析
- 加入磷酸缓冲液(PBS)以研磨已表达重组蛋白的叶子,再纯化其中的病毒样颗粒(VLP)
- 加入PBS,以溶解棕点湍蛙皮肤分泌物的冻干样本以便纯化多肽
- 加入0.1 M NaCl溶液,用于洗涤和收集青蛙的皮肤分泌物
Biochem/physiol Actions
抑制丝氨酸、半胱氨酸和金属蛋白酶。
该复合物中的组分,比如AEBSF和抑肽酶可抑制丝氨酸蛋白酶,包括胰蛋白酶、胰凝乳蛋白酶和纤溶酶等。 苯丁抑制素抑制氨肽酶。 E-64 抑制半胱氨酸蛋白酶。EDTA 是金属蛋白酶的抑制剂。 亮抑酶肽对于丝氨酸和半胱氨酸蛋白酶都可起作用。
Preparation Note
该产品以冻干粉形式提供。 一瓶制备 100 mL 混合液
一瓶可制备100mL混合液,其足以用于抑制约20克细胞提取物中的蛋白酶。
并非所有提取物都含有相同水平的内源酶,并且可能需要调整所需的混合液用量。
并非所有提取物都含有相同水平的内源酶,并且可能需要调整所需的混合液用量。
Other Notes
仅供研发使用。&不可用于药物、家庭或其他用途。有关危险和安全处理方法的信息,请参阅安全数据说明书。
该复合物组分包括:
AEBSF,2 mM
抑肽酶(Aprotinin),0.3 μM
抑氨肽素(Bestatin),116 μ
E-64,14 μM
EDTA,1 mM
亮抑酶肽(Leupeptin),1 μM
每种组分各具特定的抑制特性。
AEBSF,2 mM
抑肽酶(Aprotinin),0.3 μM
抑氨肽素(Bestatin),116 μ
E-64,14 μM
EDTA,1 mM
亮抑酶肽(Leupeptin),1 μM
每种组分各具特定的抑制特性。
Disclaimer
冻干粉末在 -20°C 可稳定保存 4 年。
signalword
Warning
hcodes
Hazard Classifications
Eye Irrit. 2 - Skin Irrit. 2
存储类别
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
低风险生物材料
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商品
While aprotinin and bovine pancreatic trypsin inhibitor (BPTI) are the same protein sequence, the term aprotinin is typically used when describing the protein derived from bovine lung.
相关内容
Select different protease inhibitor types based on your needs to prevent protein degradation during isolation and characterization and safeguard proteins in sample prep.
全球贸易项目编号
| 货号 | GTIN |
|---|---|
| P2714-30X1BTL | 04061831584950 |
| P2714-1BTL | 04061838446480 |
| SRE0055-1BTL | 04061833487488 |
| P2714-10X1BTL | 04061831584943 |
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