P2732
Monoclonal Anti-p120ctn (Catenin-related) antibody produced in mouse
clone 6H11, tissue culture supernatant
别名:
Anti-BCDS2, Anti-CAS, Anti-CTNND, Anti-P120CAS, Anti-P120CTN, Anti-p120, Anti-p120(CAS), Anti-p120(CTN)
产品名称
Monoclonal Anti-p120ctn (Catenin-related) antibody produced in mouse, clone 6H11, tissue culture supernatant
technique(s)
immunoprecipitation (IP): suitable
indirect ELISA: suitable
indirect immunofluorescence: 1:100 using cultured Madin Darby canine kidney (MDCK) epithelial cells
western blot: 1:10,000 using whole cell extract of cultured chicken fibroblasts
biological source
mouse
conjugate
unconjugated
antibody form
tissue culture supernatant
antibody product type
primary antibodies
clone
6H11, monoclonal
contains
15 mM sodium azide
species reactivity
bovine, canine, chicken, mouse, rat, human
isotype
IgG1
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Quality Level
Gene Information
human ... CTNND1(1500)
mouse ... Ctnnd1(12388)
rat ... Ctnnd1(311163)
Application
Monoclonal Anti-p120ctn (Catenin-related) antibody produced in mouse has been used in:
- immunoblotting
- enzyme linked immunosorbent assay (ELISA),
- immunocytochemistry
- immunoprecipitation
Biochem/physiol Actions
Reacts specifically with the N-terminal region of p120ctn and recognizes isoforms 1 and 2, but not isoforms 3 or 4. In immunoblotting, the antibody recognizes a 120 kDa band, and an additional very weak doublet at 200 kDa.
The 120 kD catenin-related protein p120ctn (also known as catenin p120ctn, p120cas and pp120 src substrate), was originally described as a prominent substrate for the sarcoma (Src) oncoprotein and for a variety of receptor tyrosine kinases (RTKs) including those for epidermal growth factor (EGF), platelet derived growth factor (PDGF), and colony stimulating factor-1 (CSF1). Like β-catenin and plakoglobin, it binds directly to the cytoplasmic domain of epithelial (E)-cadherin via the ARM domain and co-precipitates in multiprotein complexes containing E-cadherin or other cadherins such as neural (N)- or placental (P)- cadherins, depending on the cell type. However, unlike β-catenin and plakoglobin, p120ctn does not appear to bind α-catenin.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
General description
Monoclonal Anti-p120ctn (Catenin-related) (mouse IgG1 isotype) is derived from the 6H11 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an A/J mouse immunized with a recombinant N-terminal fragment of murine p120ctn. Catenin δ-1 (p120ctn) contains a central armadillo repeat domain (ARM domain) and is structurally similar to β-catenin and plakoglobin. At least six alternatively spliced p120ctn isoforms are generated and differentially expressed in different cell types.
Immunogen
recombinant N-terminal fragment of murine p120ctn.
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存储类别
10 - Combustible liquids
wgk
nwg
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
新产品
此项目有
J Wu et al.
Hybridoma, 17(2), 175-183 (1998-06-17)
This report describes the generation and characterization of a panel of monoclonal antibodies (MAb) to the catenin p120ctn. p120ctn (formerly p120cas) is a cadherin-binding protein with structural similarity to the classical catenins beta-catenin and plakoglobin. It was originally identified as
p120catenin alteration in cancer and its role in tumour invasion
Peglion F and Etienne-Manneville S
Philosophical transactions of the Royal Society of London. Series B, Biological sciences, 368(1629), 20130015-20130015 (2013)
p120 Catenin Associates with Microtubules INVERSE RELATIONSHIP BETWEEN MICROTUBULE BINDING AND RHO GTPase REGULATION
Franz CM and Ridley AJ
The Journal of Biological Chemistry, 279(8), 6588-6594 (2004)
Plakophilins: multifunctional scaffolds for adhesion and signaling
Bass-Zubek AE, et al.
Current Opinion in Cell Biology, 21(5), 708-716 (2009)
p120 cateninalphaN-catenin are molecular targets in the neuroprotection and neuronal plasticity mediated by atorvastatin after focal cerebral ischemia
Cespedes-Rubio A, et al.
Journal of Neuroscience Research, 88(16), 3621-3634 (2010)
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