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P2893

JumpStart^™ Taq ReadyMix^™

Name: JumpStart<SUP>™</SUP> Taq ReadyMix<SUP>™</SUP>
Brand: SIGMA

Complete optimized reagent for hot-start PCR at 2X concentration

别名:

hot start PCR master mix, hot start master mix, hot start taq master mix, mutiplex PCR

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关于此项目

UNSPSC Code:

41106300

NACRES:

NA.55

MDL number:

MFCD00166026

Concentration:

2.5 units/reaction (50 μL reaction volume)

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Quality Level

200

form

liquid

usage

sufficient for 100 reactions, sufficient for 400 reactions

feature

Multiplex PCR, dNTPs included, hotstart

concentration

2.5 units/reaction (50 μL reaction volume)

technique(s)

PCR: suitable

color

colorless

suitability

suitable for (quantitative PCR)

application(s)

agriculture

shipped in

wet ice

storage temp.

−20°C

General description

JumpStart^™ Taq ReadyMix^™是一种即用型2X预混液，其中含有JumpStart^™ Taq DNA聚合酶、99％纯dNTP、反应缓冲液和JumpStart^™抗体。Taq DNA聚合酶是一种抗体灭活的热启动酶。一旦反应温度达到70°C，DNA聚合酶-抗体复合物随即解离，Taq DNA聚合酶活性得以恢复。相对于手动热启动技术，这种抗体-酶复合物可轻松简便地准备，同时可降低污染风险。

Application

JumpStart^™ Taq ReadyMix^™ 已用于定量聚合酶链式反应(PCR)。此产品还可用于鉴定缺氧条件下的成纤维细胞表面腺苷受体(AR)类型。同时适用于：

用于需要减少非特异性扩增的 PCR 扩增
用于多重 PCR
用于减少引物二聚体

Features and Benefits

该预混液可确保反应间一致性
缩短了准备时间，并减少了多个移液步骤造成的污染风险
可最大限度减少非特异性扩增和污染
提高了特异性和靶标产量
减少了引物二聚体
与手动或蜡质热启动方法相比，准备时间更短
允许室温反应
非常适合高通量定量PCR应用

Packaging

默认反应体积为50 μL

100个反应包装为1×2.5mL
400个反应包装为1×10mL

Other Notes

2024年CiteAb奖——成功的帕金森研究供应商(2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research)

JumpStart Taq ReadyMix是一种制备好的溶液，其结合了热启动PCR的性能优势和ReadyMix的便利性。该混合物包括JumpStart Taq DNA聚合酶、99％纯脱氧核苷酸、以及2x优化的反应浓缩液缓冲液。JumpStart Taq聚合酶是一种抗体灭活的热启动酶，旨在最大限度地减少非特异性扩增，同时提高目标产量。由于JumpStart Taq聚合酶的聚合酶活性在PCR反应的第一个变性循环期间即可完全恢复，因而其与其他热启动方法（即化学灭活）不同，在循环前不需要预孵育步骤。热启动机制便于在室温进行设置，使得其成为高通量应用的理想选择。若需要制备50 μL反应，只需将25 μL ReadyMix添加至模板、引物和水中，最终反应体积为50 μL。

准备50 μL反应物，只需将 25 μL ReadyMix^™加入模板、引物和水，至终反应体积50 μL。
本品已通过定量PCR验证，但可能需要补充25 mM氯化镁溶液（货号M8787）、适宜的荧光探针以及（如果需要）内参染料（货号R4526）。请在www.sigma-aldrich.com/hotstart页面查看有关JumpStart Taq ReadyMix产品的更多详细信息。

Legal Information

本产品的使用受到如下一项或多项美国专利及其对应的境外专利声明保护：美国：US 8,404,464和US 7,972,828。根据前述专利权利要求，购买本产品包含有限不可转让的免于诉讼豁免。

JumpStart is a trademark of Sigma-Aldrich Co. LLC

ReadyMix is a trademark of Sigma-Aldrich Co. LLC

存储类别

10 - Combustible liquids

flash_point_f

Not applicable

flash_point_c

Not applicable

wgk

WGK 1

法规信息

常规特殊物品

低风险生物材料

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Although next generation sequencing (NGS) offers the potential for studying virus populations in unprecedented depth, PCR error, amplification bias and recombination during library construction have limited its use to population sequencing and measurements of unlinked allele frequencies. Here we report

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Reconfiguration of genomic anchors upon transcriptional activation of the human major histocompatibility complex.

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商品

Hot Start PCR

The purpose of Hot Start PCR is to inhibit the PCR reaction in order to reduce nonspecific amplification, prevent the formation of primer dimers, and increase product yields.

Introduction & Historical Timelines

Explore PCR's history, from discovery to Nobel Prize. Discover real-time PCR (qPCR) and digital PCR developments.

Quantitative PCR Basics

Real-time polymerase chain reaction allows researchers to estimate the quantity of starting material in a sample. It has a much wider dynamic range of analysis than conventional PCR

热启动PCR

热启动PCR的目的在于抑制PCR反应，从而减少非特异性扩增、防止引物二聚体形成并提高产物产量。

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实验方案

Antibody-Enzyme Mediated Hot Start PCR Protocol

JumpStart™ Taq DNA Polymerase is an antibody-inactivated, hot start enzyme.

End-Point PCR: Antibody-Mediated Hot Start PCR Protocol with Enhanced Specificity and Yield

Protocol using antibody mediated hot start polymerase. Method has short activation period (<1min), and results in higher yields and more specificity over standard PCR methods.

终点 PCR：特异性和产量增强的抗体介导的热启动 PCR 方案

使用抗体介导热启动聚合酶的实验方案。一种具有短活化阶段的方法（<1min), and results in higher yields and more specificity over standard PCR methods.

全球贸易项目编号

货号	GTIN
P2893-125ML	04061838262394
P2893-100RXN	04061838100610
P2893-400RXN	04061838262400
P2893-20RXN	04061833297209
P2893-50ML	04061838217745

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