P3118
Monoclonal Anti-p115/TAP antibody produced in mouse
clone 5D6, purified immunoglobulin, buffered aqueous solution
别名:
Anti-Transcytosis Associated Protein
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关于此项目
Clone:
5D6, monoclonal
Conjugate:
unconjugated
Application:
ARR, ELISA (i), ICC, IP, WB
Citations:
4
clone
5D6, monoclonal
biological source
mouse
conjugate
unconjugated
antibody form
purified immunoglobulin
antibody product type
primary antibodies
form
buffered aqueous solution
species reactivity
rat
technique(s)
immunocytochemistry: suitable, immunoprecipitation (IP): suitable, indirect ELISA: suitable, microarray: suitable, western blot: 2-4 μg/mL using cytosolic and membrane extracts of rat liver
isotype
IgG1
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Quality Level
Gene Information
rat ... Uso1(56042)
General description
Monoclonal Anti-p115/TAP (mouse IgG1 isotype) is derived from the 5D6 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from mice. p115, a Golgin family tether is also referred as TAP1 (transcytosis associated protein). It is homologous to USO1 vesicle transport factor (Uso1p). It has a coiled coil dimerization domain, an N-terminal globular domain and a C-terminal acidic domain.
Immunogen
purified rat p115/TAP.
Application
Monoclonal Anti-p115/TAP antibody produced in mouse may be used in:
- enzyme-linked immunosorbent assay (ELISA)
- immunoblotting
- immunoprecipitation
- immunocytochemistry
Biochem/physiol Actions
Monoclonal Anti-p115/TAP recognizes rat p115/TAP (approx. 95-100 kDa).
p115/TAP1 (transcytosis associated protein) plays an important role in endoplasmic reticulum to Golgi traffic and in intra−Golgi traffic. It is known to play a key role in the transport of endoplasmic reticulum (ER)/Golgi. p115 is highly essential for the docking or tethering step in intra-Golgi vesicular transport and transcytosis. It serve as a catalyst during the fusion mediated by soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE).
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, and 15 mM sodium azide.
Preparation Note
For continuous use, store at 2-8 °C for up to one month.For prolonged storage, freeze in working aliquots at −20 °C. Repeated freezing and thawing is not recom-mended. Storage in frost-free freezers is also notrecommended. If slight turbidity occurs upon prolongedstorage, clarify the solution by centrifugation beforeuse. Working dilutions should be discarded if not usedwithin 12 hours.
Disclaimer
Unless otherwise stated in our catalog, our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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此项目有
p115-SNARE Interactions: A Dynamic Cycle of p115 Binding Monomeric SNARE Motifs and Releasing Assembled Bundles
Wang T, et al.
Traffic, 16(2), 148-171 (2015)
On and off membrane dynamics of the endoplasmic reticulum-Golgi tethering factor p115 in vivo
Brandon E, et al.
Molecular Biology of the Cell, 17(7), 2996-3008 (2006)
Binding relationships of membrane tethering components The giantin N terminus and the GM130 N terminus compete for binding to the p115 C terminus
Linstedt AD, et al.
The Journal of biological chemistry, 275(14), 10196-10201 (2000)
Gabriella Frigerio et al.
Traffic (Copenhagen, Denmark), 8(11), 1644-1655 (2007-09-01)
ADP-ribosylation factors (ARFs) are critical regulators of vesicular trafficking pathways and act at multiple intracellular sites. ADP-ribosylation factor-GTPase-activating proteins (ARFGAPs) are proposed to contribute to site-specific regulation. In yeast, two distinct proteins, Glo3p and Gcs1p, together provide overlapping, essential ARFGAP
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