P3201
Platelet-Derived Growth Factor-BB 人
≥98% (HPLC and SDS-PAGE), recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture
别名:
PDGF-BB
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关于此项目
Biological source:
human
Recombinant:
expressed in E. coli
Assay:
≥98% (HPLC and SDS-PAGE)
Form:
lyophilized powder
Mol wt:
~25 kDa
Impurities:
≤1 EU/μg
产品名称
Platelet-Derived Growth Factor-BB 人, PDGF-BB, recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture
biological source
human
Quality Segment
recombinant
expressed in E. coli
assay
≥98% (HPLC and SDS-PAGE)
form
lyophilized powder
potency
1.0-3.0 ng/mL EC50
quality
endotoxin tested
mol wt
~25 kDa
packaging
pkg of 10 μg, pkg of 50 μg
storage condition
avoid repeated freeze/thaw cycles
technique(s)
cell culture | mammalian: suitable
impurities
≤1 EU/μg
UniProt accession no.
storage temp.
−20°C
Gene Information
human ... PDGFB(5155)
General description
Recombinant human PDGF‑BB is a homodimeric growth factor expressed in E. coli and supplied as a lyophilized powder for mammalian cell culture research applications in vitro.
Application
Platelet-Derived Growth Factor-BB human has been used :
- As a growth supplement in proximal tubule and glomerular explant cell culture
- In three-dimensional scaffold preparation
- To study the effect of brivanib on growth factor signaling pathways
- In transwell migration assay
Biochem/physiol Actions
Platelet-derived growth factor (PDGF) elicits multifunctional actions with a variety of cells. It is mitogenic to mesoderm-derived cells, such as dermal and tendon fibroblasts, vascular smooth muscle cells, glial cells, and chondrocytes. PDGF significantly activates chemotaxis of neutrophils, monocytes, and fibroblasts. It increases the synthesis of phospholipids, cholesterol esters, glycogen and prostaglandins, and modulates LDL (low density lipoprotein) receptor binding.
PDGF stimulates fibroblast proliferation and extracellular matrix production in order to facilitate tissue repair. PDGF-BB isomer is found to be critical in angiogenesis and is highly expressed in tumor, mediating oncogenic pericyte recruitment. PDGF-BB is an important regulator of bone homeostasis, repair and regeneration.
PDGF stimulates fibroblast proliferation and extracellular matrix production in order to facilitate tissue repair. PDGF-BB isomer is found to be critical in angiogenesis and is highly expressed in tumor, mediating oncogenic pericyte recruitment. PDGF-BB is an important regulator of bone homeostasis, repair and regeneration.
Physical form
Lyophilized from a 0.2 μm filtered solution in 30 mM acetic acid.
Analysis Note
The biological activity is measured by its ability to stimulate 3H-thymidine incorporation in quiescent NR6R-3T3 fibroblasts.
Other Notes
Dimer of the B chain of human PDGF.
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存储类别
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)