P3296
蛋白G琼脂糖凝胶™，快速流动
recombinant, expressed in E. coli, aqueous ethanol suspension
别名:
蛋白G琼脂糖，快速流动 来源于链球菌 属
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关于此项目
Form:
aqueous ethanol suspension
Recombinant:
expressed in E. coli
recombinant
expressed in E. coli
Quality Level
form
aqueous ethanol suspension
analyte chemical class(es)
proteins (Immunoglobulins of various mammalian species)
extent of labeling
~2 mg per mL
technique(s)
affinity chromatography: suitable
matrix
Sepharose 4B Fast Flow
matrix activation
cyanogen bromide
matrix attachment
amino
matrix spacer
1 atom
storage temp.
2-8°C
General description
蛋白G是从G组链球菌菌株G-148中分离得到的细菌胞壁蛋白，它能与免疫球蛋白（IgG）结合。通过木瓜蛋白酶消化从细胞中提取该蛋白，并依次使用DEAE-Sephadex离子交换层析、琼脂糖凝胶偶联人IgG亲和层析以及Sephadex G-200凝胶层析进行纯化。蛋白G与各种多克隆和单克隆IgG结合的pH条件基本在2.8-10之间，在pH 4-5时结合最强，在pH 10时结合最弱。它是强大的IgG检测试剂。
P3296-5Ml的最新产品编号为GE17-0618-01
P3296-5Ml的最新产品编号为GE17-0618-01
Application
蛋白G琼脂糖凝胶™ 被用于亲和层析，蛋白质层析，抗体纯化和表征，免疫亲和基质，蛋白A、G和L树脂，蛋白质相互作用以及纯化和检测。蛋白G琼脂糖凝胶™ 已被用于开发一种可确认人血清中是否存在抗促红细胞生成素中和抗体的方法，以及比较马血清中白蛋白和IgG消耗的方法。
Physical form
悬浮于20%乙醇中
Preparation Note
使用重组链球菌蛋白G制备，其中白蛋白结合区已被移除
Legal Information
Sepharose is a trademark of Cytiva
signalword
Warning
hcodes
Hazard Classifications
Flam. Liq. 3
存储类别
3 - Flammable liquids
wgk
WGK 3
flash_point_f
115.0 °F - closed cup
flash_point_c
46.1 °C - closed cup
法规信息
常规特殊物品
此项目有
实验方案
Techniques for protein antigen molecular weight determination, protein interactions, enzymatic activity, and post-translational modifications.
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全球贸易项目编号
| 货号 | GTIN |
|---|---|
| P3296-2ML | 04061838249630 |
| P3296-5ML | 04061838087539 |
| P3296-1ML | 04061838099730 |